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Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation
Published on: November 26, 2018
[Aberrant immunophenotypes in 126 patients with acute leukemia]
Jing-Juan He1, Yao-Hua Li, Xue-Jing Sun
1Department of Hematology, Xuanwu Hospital of Capital University of Medical Sciences, Beijing 100053, China.
Insights
Leukemia aberrant immunophenotypes (LAIP) are key for detecting minimal residual disease (MRD) in acute leukemia. This study found LAIP in 76% of patients, confirming immunophenotyping
Area of Science:
- Hematology
- Immunology
- Oncology
Background:
- Minimal residual disease (MRD) detection is crucial in acute leukemia management.
- Leukemia aberrant immunophenotypes (LAIP) offer a potential method to distinguish leukemic cells from normal hematopoietic progenitors.
Purpose of the Study:
- To analyze the characteristics of LAIP in acute leukemia.
- To determine the prevalence of different LAIP types in acute leukemia patients.
Main Methods:
- Flow cytometry (FCM) utilizing four-color analysis and CD45/SSC gating.
- Analysis of bone marrow samples from 126 acute leukemia patients.
Main Results:
- Definite LAIP were detected in approximately 76% of patients.
- Four main LAIP categories were identified: cross-lineage antigen expression (39%), asynchronous antigen expression (46%), antigen overexpression (21%), and antigen lack expression (29%).
- Multiple aberrant phenotypes were observed in the majority of cases.
Conclusions:
- LAIP, categorized into four subgroups, are prevalent in most acute leukemia patients.
- Immunophenotyping based on LAIP is a viable approach for MRD detection in acute leukemia.
Abstract:
The existence of leukemia aberrant immunophenotypes (LAIP) has been suggested to be a valuable tool for the detection of minimal residual disease (MRD), as they could distinguish leukemic cells from normal hematopoietic progenitors. This study was purposed to analyze the characteristics of LAIP in acute leukemia and further explore the proportion of different types of LAIP in acute leukemia patients. Flow cytometry (FCM) with four color and CD45/SSC gating were used to detect the antigen expression in samples of bone marrow from 126 patients with acute leukemia. The results showed that definite LAIP could be detected in about 76% patients. The LAIP could be divided into four groups as cross-lineage antigen expression, asynchronous antigen expression, antigen overexpression and antigen lack expression. The percentages of these LAIPs were 39%, 46%, 21% and 29% respectively. About 11% out of analyzed cases showed the existence of only one aberrant phenotype while two or more of aberrant phenotypes could be detected in majority cases. It is concluded that the LAIP with four subgroups can be detected in the majority of patients with acute leukemia and immunophenotyping based on LAIP is applicable for the detection of MRD.
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