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Updated: Jul 8, 2026

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
09:09

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

Published on: April 18, 2016

CD8- dendritic cells preferentially cross-present Saccharomyces cerevisiae antigens

Ronald Backer1, Fred van Leeuwen, Georg Kraal

  • 1Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, The Netherlands.

European Journal of Immunology
|January 18, 2008
PubMed

Insights

Different mouse dendritic cell (DC) subsets show distinct roles in immune responses. CD8(-) DCs, but not CD8(+) DCs, effectively activate CD8(+) T cells and produce key cytokines when encountering fungal antigens.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Dendritic cell (DC) subsets have specialized functions in antigen presentation.
  • CD8(+) DCs excel at cross-presentation to CD8(+) T cells.
  • CD8(-) DCs are more efficient in presenting antigens to CD4(+) T cells.

Purpose of the Study:

  • To investigate the distinct capacities of CD8(+) and CD8(-) DC subsets in presenting fungal antigens.
  • To analyze the presentation of fungal antigens via MHC class I and II molecules.
  • To determine the impact on CD8(+) and CD4(+) T cell responses.

Main Methods:

  • Utilized ovalbumin-expressing Saccharomyces cerevisiae (yeast-OVA) as a fungal model.
  • Assessed phagocytosis of yeast by DC subsets mediated by dectin-1.
  • Measured T cell proliferation and activation.
  • Quantified cytokine production and mRNA levels.

Main Results:

  • Both CD8(+) and CD8(-) DC subsets exhibited equal yeast phagocytosis via dectin-1.
  • Both DC subsets induced similar OVA-specific CD4(+) T cell proliferation.
  • OVA-specific CD8(+) T cell activation was primarily induced by CD8(-) DCs.
  • CD8(-) DCs produced IL-10, TNF-alpha, and increased IL-23 mRNA levels in response to yeast.

Conclusions:

  • Dendritic cell subsets display differential functions in initiating adaptive immunity.
  • CD8(-) DCs play a crucial role in CD8(+) T cell activation against fungal antigens.
  • Distinct cytokine profiles of DC subsets contribute to varied immune responses.

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