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Gamma-interferon promotes the release of IgE-binding factors (soluble CD23) by human epidermal Langerhans cells

T Bieber1, G Delespesse

  • 1Department of Dermatology, University of Munich, Germany.

The Journal of Investigative Dermatology
|September 1, 1991
PubMed

Insights

Human epidermal Langerhans cells (LC) express Fc epsilon R2/CD23 and release IgE-BF (soluble CD23) when stimulated with IL-4 or IFN-gamma. These cytokines differentially regulate LC responses.

Area of Science:

  • Immunology
  • Dermatology

Background:

  • Human epidermal Langerhans cells (LC) are key immune cells in the skin.
  • Previous studies indicated LC can express Fc epsilon R2/CD23 upon stimulation with IL-4 and/or IFN-gamma.

Purpose of the Study:

  • To investigate the role of IL-4 and IFN-gamma in Fc epsilon R2/CD23 expression and IgE-BF release by human LC.
  • To differentiate the effects of IL-4 and IFN-gamma on LC responses.

Main Methods:

  • Utilized LC-enriched and LC-depleted epidermal cell (EC) cultures.
  • Stimulated cells with Interleukin-4 (IL-4) and/or Interferon-gamma (IFN-gamma).
  • Quantified Fc epsilon R2/CD23 expression and IgE-BF release.

Main Results:

  • IL-4 and IFN-gamma stimulation induced Fc epsilon R2/CD23 expression and IgE-BF release in human LC.
  • IL-4 was more effective in inducing Fc epsilon R2/CD23 expression on LC.
  • IFN-gamma strongly promoted IgE-BF release from LC.

Conclusions:

  • Fc epsilon R2/CD23-positive LC are a potential source of IgE-BF.
  • IFN-gamma and IL-4 differentially regulate Fc epsilon R2/CD23 expression and IgE-BF release on human LC, similar to monocytes.

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