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Isolation of Lamina Propria Mononuclear Cells from Murine Colon Using Collagenase E
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Isolation of Lamina Propria Mononuclear Cells from Murine Colon Using Collagenase E

Published on: September 26, 2019

Human colonic intraepithelial lymphocytes regulate the cytokines produced by lamina propria mononuclear cells

P Hoang1, J P Dehennin, L Li

  • 1Department of Gastroenterology University Hospital St-Luc Brussels B-1200 Belgium.

Mediators of Inflammation
|January 1, 1997
PubMed

Insights

Colonic intraepithelial lymphocytes (IEL) can enhance cytokine production by lamina propria mononuclear cells (LPMNC) through soluble factors, not cell contact. This finding is crucial for understanding gastrointestinal mucosal immune activation.

Area of Science:

  • Immunology
  • Gastroenterology
  • Cell Biology

Background:

  • Colonic intraepithelial lymphocytes (IEL) and lamina propria mononuclear cells (LPMNC) are key immune cells in the gut mucosa.
  • The specific immunomodulatory roles of IEL on LPMNC cytokine production require further elucidation.

Purpose of the Study:

  • To investigate the immunomodulatory function of colonic IEL on cytokine production by LPMNC in an in vitro autologous human system.
  • To determine if IEL influence cytokine synthesis through direct cell-to-cell contact or soluble factors.

Main Methods:

  • In vitro autologous human system utilizing co-culture of IEL and LPMNC.
  • Transwell filter culture system to differentiate between cell contact-dependent and independent mechanisms.
  • Measurement of cytokine production (IL-10, interferon-gamma, IL-2) using PHA-induced stimulation.

Main Results:

  • Colonic IEL produced low levels of IL-10, interferon-gamma, and IL-2 compared to LPMNC.
  • Co-culture of IEL and LPMNC enhanced PHA-induced IL-2 and interferon-gamma synthesis by LPMNC, but not IL-10.
  • The enhancing effect of IEL on LPMNC cytokine production was independent of direct cell-to-cell contact.

Conclusions:

  • Colonic IEL modulate LPMNC cytokine synthesis, primarily through the release of soluble factors.
  • These findings suggest a significant role for IEL-derived soluble mediators in regulating immune responses within the gastrointestinal mucosa.
  • This study provides insights into the in vivo immune activation mechanisms of the gut mucosa.

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