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Updated: Jul 4, 2026
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
Published on: March 26, 2018
Study of morphocytochemical and immunophenotypic features of acute leukemia stem cells
D F Gluzman1, V A Nadgornaya, L M Sklyarenko
1R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine. vals@onconet.kiev.ua.
Insights
New immunophenotypic markers can improve acute leukemia classification. Studies suggest a common precursor for certain leukemias, potentially explaining AML recurrence after pro-B-ALL.
Area of Science:
- Hematology
- Immunology
- Oncology
Background:
- Hematopoietic stem cells (HSC), hematopoietic precursor cells, and leukemic stem cells (LSC) immunophenotypes are widely studied.
- Accurate classification of acute leukemias is crucial for effective treatment.
Purpose of the Study:
- To identify novel immunophenotypic markers for precise acute leukemia classification.
- To investigate potential common cellular origins for related leukemia subtypes.
- To explore the relationship between pro-B acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML).
Main Methods:
- Immunophenotypic analysis of hematopoietic and leukemic stem cells.
- Cytogenetic analysis of blast cells.
- Comparative analysis of cell surface markers and genetic abnormalities.
Main Results:
- Proposed new markers to supplement standard panels for classifying acute leukemias, distinguishing closely related forms like AML M6b and AML M7.
- Identified a potential common bipotent LSC in AML M7 and AML M6b, analogous to megakaryopoiesis and erythropoiesis precursors.
- Observed immunophenotypic and cytogenetic similarities between pro-B-ALL [t(4;11), 11q23] and AML M5a [t(9;11), 11q23] blast cells.
Conclusions:
- Supplementing standard acute leukemia classification panels with new markers can enhance diagnostic precision.
- A common LSC may underlie AML M7 and AML M6b, suggesting shared developmental pathways.
- Immunophenotypic and cytogenetic similarities between pro-B-ALL and AML M5a blast cells may explain AML M5a recurrence after initial pro-B-ALL diagnosis.
Abstract:
The immunophenotypic profile of hematopoietic stem cells (HSC) and hematopoietic precursor cells as well as leukemic stem cells (LSC) has been extensively studied in several laboratories worldwide. The results of our studies suggest that the standard panel for classification of acute leukemias should be supplemented with several new markers allowing us to identify more precisely the different forms of the leukemias being of the closely related origin, for example AML M6b and AML M7. The common bipotent LSC in AML M7 of low grade and AML M6b may exist analogous to precursor cell common for megakaryocytopoiesis and erythropoiesis. We have also found the similarity between blast cells in pro-B-ALL [t (4;11), 11q23] and AML M5a [t (9;11), 11q23]. Such similarity of immunophenotype and cytogenetic abnormalities in blast cells in pro-B-ALL and AML M5a may be considered as hint explaining the cases of AML M5a as a recurrence of leukemia in children with originally diagnosed pro-B-ALL.
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