Effect of temperature on hybridoma cell cycle and MAb production

Area of Science:

• Biotechnology
• Cell Biology
• Immunology

Background:

• Hybridoma cell lines are crucial for monoclonal antibody production.
• Understanding the impact of environmental factors like temperature on cell growth and antibody formation is vital for optimizing bioprocesses.

Purpose of the Study:

• To investigate the effect of varying temperatures (34°C–39°C) on the growth kinetics and antibody production of an anti-interleukin-2 producing hybridoma.
• To determine the optimal temperature for maximizing cell density and monoclonal antibody yield.

Main Methods:

• Suspension culture of a hybridoma cell line.
• Temperature variation from 34°C to 39°C.
• Flow cytometry to analyze cell cycle distribution.
• Measurement of cell density and monoclonal antibody concentration.

Main Results:

• Maximum cell density and monoclonal antibody yield were achieved at 37°C.
• Lower temperatures (below 37°C) led to prolonged G1-phase duration in the cell cycle.
• Specific monoclonal antibody production rate remained relatively constant across tested temperatures.
• Antibody production was found to be directly correlated with viable cell concentration.

Conclusions:

• The optimal temperature for growth and antibody production in this anti-IL-2 hybridoma is 37°C.
• Temperature-induced cell cycle arrest in the G1-phase does not enhance monoclonal antibody production.
• Viable cell concentration is the primary determinant of antibody yield for this hybridoma.