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A Preclinical Controlled Cortical Impact Model for Traumatic Hemorrhage Contusion and Neuroinflammation
06:50

A Preclinical Controlled Cortical Impact Model for Traumatic Hemorrhage Contusion and Neuroinflammation

Published on: June 10, 2020

Intracerebral hemorrhage leads to infiltration of several leukocyte populations with concomitant pathophysiological

Matthew C Loftspring1, Jeremiah McDole, Aigang Lu

  • 1Department of Neurology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0536, USA.

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
|October 2, 2008
PubMed

Insights

Intracerebral hemorrhage (ICH) triggers immune cell infiltration in the brain. This study reveals a novel CD4 T-cell response, suggesting adaptive immunity plays a role in brain injury after ICH.

Area of Science:

  • Neuroscience
  • Immunology
  • Stroke Research

Background:

  • Intracerebral hemorrhage (ICH) is a severe stroke subtype with high mortality and morbidity.
  • The roles of complement and cytokine signaling in ICH-induced brain injury are known, but cellular immunology remains understudied.
  • Understanding immune cell dynamics post-ICH is crucial for developing targeted therapies.

Purpose of the Study:

  • To quantitatively profile immune cell populations infiltrating the brain after ICH using flow cytometry.
  • To identify specific immune cell types and their changes in response to ICH.
  • To investigate the potential role of adaptive immunity in the ICH response.

Main Methods:

  • Utilized flow cytometry to analyze immune cell infiltration in the brain at 1 and 4 days post-ICH in a mouse model.
  • Quantitatively measured changes in various immune cell populations, including CD45(hi) GR-1(+) and CD4(+) cells.
  • Compared immune cell profiles between ICH mice and saline-treated controls.

Main Results:

  • At 1 day post-ICH, a significant increase in CD45(hi) GR-1(+) cells was observed.
  • At 4 days post-ICH, significant increases in CD45(hi) cells, CD45(hi) GR-1(-) cells, CD45(hi) GR-1(+) cells, and notably, CD4(+) T-cells were detected.
  • No significant changes were found in CD8(+) cells or CD45(lo) GR-1(-) cells.

Conclusions:

  • This study represents the first use of flow cytometry to analyze leukocyte infiltration following ICH.
  • The identification of a CD4 T-cell infiltrate post-ICH is a novel finding.
  • These results suggest a significant role for the adaptive immune system in the brain's response to intracerebral hemorrhage.

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