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Elevated resting and agonist-induced whole blood chemiluminescence in patients with active infective endocarditis

Stanislaw Ostrowski1, Marek Kasielski, Jacek Kordiak

  • 1Departament of Cardiosurgery, Medical University of Lodz, Poland.

Interactive Cardiovascular and Thoracic Surgery
|October 3, 2008
PubMed

Insights

Patients with infective endocarditis (IE) show higher oxidant production by phagocytes, measured by luminol-enhanced whole blood chemiluminescence (LBCL). This elevated oxidative activity may increase the risk of oxidative stress in IE patients.

Area of Science:

  • Immunology
  • Cardiovascular Medicine
  • Biochemistry

Background:

  • Infective endocarditis (IE) and cardiac surgery involve inflammatory responses impacting phagocyte oxidant production.
  • Phagocyte-derived oxidants play a role in inflammation and tissue damage.

Purpose of the Study:

  • To evaluate luminol-enhanced whole blood chemiluminescence (LBCL) as a measure of oxidative production by circulating phagocytes.
  • To compare oxidative production in IE patients, valvular heart disease patients, and healthy controls.

Main Methods:

  • LBCL was measured in 26 IE patients, 27 valvular heart disease patients, and 25 healthy controls.
  • Blood samples were collected pre-surgery and at multiple time points post-valve replacement.
  • Resting (rCL) and stimulated (fMLP-induced, tCL) chemiluminescence were assessed.

Main Results:

  • Preoperative LBCL (rCL and tCL) was significantly higher in IE patients compared to healthy controls and valvular heart disease patients.
  • IE patients exhibited approximately 2.5-fold higher median preoperative rCL and tCL than valvular heart disease patients.
  • LBCL increased significantly in both operated groups three days post-surgery, decreasing with recovery.

Conclusions:

  • Infective endocarditis is associated with elevated LBCL, indicating increased oxidant release from circulating phagocytes.
  • Elevated phagocyte oxidant production in IE may contribute to the development of oxidative stress.
  • LBCL serves as a valuable indicator of phagocyte oxidative activity in cardiovascular conditions.

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