Related Experiment Video

Updated: Jun 26, 2026

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1
07:42

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1

Published on: February 28, 2025

Augmented chemokine levels and chemokine receptor expression on immune cells during pulmonary tuberculosis

Supriya Pokkali1, Sulochana D Das

  • 1Department of Immunology, Tuberculosis Research Centre (ICMR), Chennai, India.

Human Immunology
|December 23, 2008
PubMed

Insights

Mycobacterium tuberculosis infection alters immune mediators, affecting cell movement. This study reveals how specific chemokines and cytokines change, influencing immune cell trafficking in pulmonary tuberculosis patients.

Area of Science:

  • Immunology
  • Infectious Diseases
  • Molecular Biology

Background:

  • Mycobacterium tuberculosis (M. tuberculosis) infection causes systemic immune changes.
  • Cytokines and chemokines are key mediators of immune cell trafficking.
  • Understanding these interactions is crucial for managing pulmonary tuberculosis (PTB).

Purpose of the Study:

  • To investigate systemic changes in immune mediators during PTB.
  • To analyze the synchronized interaction of cytokines and chemokines.
  • To assess their role in regulating immune cell trafficking in PTB.

Main Methods:

  • Cytometric Bead Array (CBA) and ELISA for cytokine/chemokine quantification.
  • Flow cytometry for chemokine receptor expression analysis.
  • Study included 34 PTB patients and 30 healthy controls.

Main Results:

  • Increased levels of IP-10, MIG, IL-8, RANTES, and IL-6 observed in PTB.
  • Decreased levels of Interferon-gamma, TNF-alpha, and TGF-beta in PTB.
  • Differential expression of chemokine receptors (CCR1, CCR2, CXCR2, CCR7, CXCR3) on immune cells in PTB patients.

Conclusions:

  • Chemokines function coordinately and consistently during PTB.
  • Peripheral chemokine-cytokine balance influences effector immune cell trafficking.
  • Differential chemokine receptor expression may modulate monocyte migration in PTB.

More Related Videos

A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection10:10

A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection

Published on: October 5, 2015

Related Concept Videos

Pulmonary Tuberculosis II01:28

Pulmonary Tuberculosis II

Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
Tuberculosis01:23

Tuberculosis

Tuberculosis (TB) remains a significant global health concern, primarily targeting the lungs and spreading through airborne transmission. Infection begins when aerosolized droplet nuclei, expelled by an individual with active TB, are inhaled by another person. These microscopic particles carry Mycobacterium tuberculosis, the causative agent of TB. Upon reaching the alveoli, the bacilli are engulfed by alveolar macrophages. However, due to their specialized lipid-rich cell wall, these pathogens...
Pulmonary Tuberculosis I01:29

Pulmonary Tuberculosis I

Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Pulmonary Tuberculosis III01:31

Pulmonary Tuberculosis III

Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
Pulmonary Tuberculosis V01:28

Pulmonary Tuberculosis V

Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the progression...