Related Experiment Video

Updated: Aug 9, 2026

The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis
23:06

The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis

Published on: August 11, 2008

Rapid serodiagnostic test for pulmonary tuberculosis

V V Radhakrishnan1, A Mathai, B Sunderam

  • 1Department of Pathology, Institute for Medical Sciences and Technology, Trivandrum, India.

Indian Journal of Experimental Biology
|May 1, 1991
PubMed

Insights

Counter-current immunoelectrophoresis (CIE) detected specific mycobacterial antibodies in immune complexes (ICs) from pulmonary tuberculosis (PT) patients. This simple test is valuable for diagnosing PT, especially when sputum tests are negative.

Area of Science:

  • Immunology
  • Medical Diagnostics
  • Microbiology

Background:

  • Immune complexes (ICs) play a role in various diseases.
  • Diagnosing pulmonary tuberculosis (PT) can be challenging, particularly with negative sputum bacteriology.

Purpose of the Study:

  • To investigate the presence of specific mycobacterial antibodies in ICs of PT patients.
  • To evaluate counter-current immunoelectrophoresis (CIE) as a potential serodiagnostic tool for PT.

Main Methods:

  • Immune complexes were isolated from patient and control sera using polyethylene glycol (PEG) precipitation.
  • Specific mycobacterial antibodies within ICs were detected using counter-current immunoelectrophoresis (CIE) against a purified mycobacterial antigen.

Main Results:

  • Specific mycobacterial antibodies were found in the ICs of 70% of PT patients.
  • No specific antibodies were detected in ICs from non-tuberculous pulmonary disease patients or healthy controls.

Conclusions:

  • CIE is a sensitive and rapid method for detecting specific mycobacterial antibodies in PT patients' ICs.
  • CIE offers advantages over ELISA and is suitable for resource-limited laboratories for PT diagnosis.

More Related Videos

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test08:10

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test

Published on: April 9, 2012

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection09:54

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection

Published on: February 16, 2020

Related Concept Videos

Pneumonia III: Complications and Assessment01:30

Pneumonia III: Complications and Assessment

Pneumonia poses the potential for numerous complications that warrant consideration. These complications include the following:
Pulmonary Tuberculosis I01:29

Pulmonary Tuberculosis I

Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
Pulmonary Tuberculosis II01:28

Pulmonary Tuberculosis II

Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
Pulmonary Tuberculosis III01:31

Pulmonary Tuberculosis III

Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Pulmonary Tuberculosis V01:28

Pulmonary Tuberculosis V

Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the progression...