Related Experiment Video

Updated: Jun 25, 2026

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Sandwich-type electrochemiluminescence immunosensor based on N-(aminobutyl)-N-ethylisoluminol labeling and gold

Dayong Tian1, Chunfeng Duan, Wei Wang

  • 1Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.

Talanta
|February 11, 2009
PubMed

Insights

A new electrochemiluminescence immunosensor detects human immunoglobulin G (hIgG) using ABEI labeling. This sensitive method offers reliable detection of hIgG in human serum samples.

Area of Science:

  • Analytical Chemistry
  • Biomedical Engineering
  • Immunotechnology

Background:

  • Human immunoglobulin G (hIgG) is a crucial biomarker in various physiological and pathological conditions.
  • Sensitive and specific detection methods for hIgG are essential for accurate disease diagnosis and monitoring.
  • Existing detection techniques may lack the sensitivity, speed, or cost-effectiveness required for widespread clinical application.

Purpose of the Study:

  • To develop a novel electrochemiluminescence (ECL) sandwich-type immunosensor for the sensitive detection of human immunoglobulin G (hIgG).
  • To utilize N-(aminobutyl)-N-ethylisoluminol (ABEI) labeling and a gold nanoparticle modified electrode for enhanced signal generation.
  • To evaluate the performance of the developed immunosensor for hIgG detection in biological samples, specifically human serum.

Main Methods:

  • Fabrication of a gold nanoparticle modified electrode.
  • Immobilization of goat-anti-human IgG as the primary antibody.
  • Conjugation of human IgG (antigen) and ABEI-labeled secondary antibody to form a sandwich immunocomplex.
  • Electrochemical analysis using a double-step potential in carbonate buffer solution with hydrogen peroxide as a co-reactant.

Main Results:

  • The developed immunosensor exhibited a linear response to hIgG concentration in the range of 5.0-100 ng/mL.
  • A low limit of detection (LOD) of 1.68 ng/mL (S/N=3) was achieved, indicating high sensitivity.
  • The immunosensor demonstrated good reproducibility with a relative standard deviation of 3.79% at 60 ng/mL (n=9).
  • Successful application of the immunosensor for the detection of hIgG in human serum samples was confirmed.

Conclusions:

  • A novel, simple, and highly sensitive ECL sandwich-type immunosensor for hIgG detection was successfully developed.
  • The use of ABEI labeling and gold nanoparticle modification significantly enhances the sensor's performance.
  • The developed immunosensor shows great potential for the accurate and efficient detection of hIgG in clinical diagnostics.

More Related Videos

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds10:10

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...