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Localization of p62c-yes protein in mammalian neural tissues
Y H Zhao1, H Baker, S I Walaas
1Laboratory of Molecular Oncology, Rockefeller University, New York, New York 10021.
Insights
The c-yes proto-oncogene, p62c-yes, is highly expressed in specific brain regions, retina, and adrenal gland in mammals. This mapping provides new systems to study its function in neural processes.
Area of Science:
- Neuroscience
- Oncology
Background:
- The c-yes proto-oncogene encodes a protein tyrosine kinase involved in cellular signaling.
- Its expression and function in mammalian neural tissues are not fully understood.
Purpose of the Study:
- To investigate the expression pattern of the c-yes proto-oncogene (p62c-yes) in mammalian brain, retina, and adrenal gland.
- To identify specific cell types and brain regions where c-yes is highly expressed.
Main Methods:
- Immunohistochemistry using affinity-purified anti-yes IgG.
- Immune assays to detect and map p62c-yes protein.
Main Results:
- High p62c-yes expression was observed in olfactory bulb mitral cells, cerebellar Purkinje cells, hippocampal neurons, dentate gyrus granule neurons, and ependymal cells.
- Intense immunoreactivity was also found in retinal ganglion cells, the inner segment layer of photoreceptors, and adrenal medulla cells.
- Lower levels of c-yes were detected in most other neuronal cell bodies.
Conclusions:
- The study maps p62c-yes expression to distinct areas within the mammalian central nervous system, retina, and adrenal gland.
- These findings highlight potential experimental systems for investigating the role of c-yes in neural functions.
Abstract:
Expression of the c-yes proto-oncogene in mammalian brain, retina and adrenal gland was studied by immunohistochemistry and immune assays with affinity-purified anti-yes IgG. Immunohistochemical staining with anti-yes IgG showed that in the central nervous system p62c-yes is highly expressed in mitral cells of the olfactory bulb, Purkinje cells of the cerebellum, hippocampal neurons and granule neurons of the dentate gyrus and ependymal cells lining central nervous system ventricles. Less intense labeling by anti-yes IgG was observed in most neuronal cell bodies in the brain. In addition, we observed intense c-yes immunoreactivity in the ganglion cells of the retina, the inner segment layer of rods and cones and medullary cells of the adrenal gland. Mapping p62c-yes expression to specific areas of mammalian neural tissues points to attractive experimental systems which could be used to investigate the function of the proto-oncogene product in neural processes.
