Related Experiment Video
Updated: Jun 25, 2026
Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation
Published on: November 26, 2018
[Analysis of immunophenotype, lymphocytic subsets and NK cells in patients with B cell chronic lymphoid leukemia]
Li Zhang1, Lei Zhu, Hong-Wei Wang
1Department of Hematology, The Second Clinical Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China.
Insights
Immunophenotyping of leukemic cells in bone marrow and peripheral blood aids in diagnosing B cell chronic lymphoid leukemia (B-CLL). Flow cytometry reveals specific cell subset changes crucial for patient management.
Area of Science:
- Hematology
- Immunology
- Oncology
Context:
- B cell chronic lymphoid leukemia (B-CLL) is a lymphoid malignancy.
- Accurate diagnosis and prognosis rely on detailed cellular analysis.
- Multi-parameter flow cytometry is a key diagnostic tool in hematologic malignancies.
Purpose:
- To evaluate the diagnostic, therapeutic, and prognostic value of immunophenotyping.
- To analyze lymphocytic subsets and NK cells in peripheral blood (PB) and bone marrow (BM) of B-CLL patients.
- To correlate specific immunophenotypic markers with disease characteristics.
Summary:
- A study of 67 B-CLL patients using multi-parameter flow cytometry showed increased PB lymphocytes.
- Significant decreases in CD3, CD4, CD8, and NK cells were observed, with no change in the CD4/CD8 ratio.
- High expression rates of CD19 (91.04%), CD5 (80.60%), and CD20 (76.12%) were noted, along with correlations between ZAP-70 and CD38 expression.
Impact:
- Immunophenotyping of leukemic cells, PB lymphocytic subsets, and PB NK cells aids in B-CLL diagnosis.
- Findings support the utility of flow cytometry in characterizing B-CLL.
- This analysis contributes to a better understanding of B-CLL cell biology and potential therapeutic targets.
Abstract:
The aim of this study was to evaluate the values of immunophenotype of leukemic cells in bone marrow (BM) or peripheral blood (PB), lymphocytic subsets and NK cells of PB in diagnosis, therapy and prognosis of patients with B cell chronic lymphoid leukemia (B-CLL). The samples of PB and BM from 67 patients with B-CLL were collected and detected by multi-parameter flow cytometry. The results showed that the percentage of lymphocytes in PB of 67 patients with B-CLL obviously increased, as compared with normal persons, while CD3, CD4, CD8 and NK cells decreased significantly (p < 0.01), and no CD4/CD8 ratio had notable change (p > 0.05). In their immunophenotype results, the positive expression rate of CD19 was highest (91.04%), followed by CD5 (80.60%), CD 20 (76.12), cyCD 79a (74.63%), CD 38 (43.28%), CD 11c (42.86%), CD 7 (41.94%), ZAP-70 (39.29%), CD25 (0.00%); co-expression rate of CD5 and CD19 was 72.73%; the expression of ZAP-70 was correlated with CD 38 (p < 0.05). In conclusion, detection of cell immunophenotypes, lymphocytic subsets in PB and BM and NK cells in PB contribute to diagnosis of patients with B-CLL.
More Related Videos
09:43An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection
Published on: March 30, 2018
11:39Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking
Published on: October 23, 2019