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Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
10:10

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

[An experimental luminescent immunochromatographic system]

S P Iarkov, S N Skopinskaia, I V Shilenko

    Vestnik Rossiiskoi Akademii Meditsinskikh Nauk
    |April 24, 2009
    PubMed

    Insights

    A novel luminescent latex assay offers a sensitive method for detecting microbial antigens and toxins. This immunochromatographic assay demonstrates superior performance compared to existing nanoparticle-based detection methods.

    Area of Science:

    • Biotechnology
    • Analytical Chemistry
    • Immunology

    Background:

    • Immunochromatographic assays are widely used for detecting various analytes.
    • Current methods often rely on colloidal gold or quantum dots, which have limitations in sensitivity and signal stability.
    • There is a need for more sensitive and efficient detection systems for microbial antigens and toxins.

    Purpose of the Study:

    • To develop a highly sensitive luminescent immunochromatographic assay for the detection of microbial antigens, cells, and toxins.
    • To evaluate the performance of luminescent submicrone latex particles as a detection label in immunochromatographic procedures.
    • To compare the efficacy of luminescent latexes with other labels like quantum dots, liposomes, and colloidal gold.

    Main Methods:

    • Development of a luminescent immunochromatographic assay utilizing luminescent submicrone latex particles conjugated with antibodies.
    • Preparation of luminescent mobile phases including quantum labels, ganglioside-containing liposomes with sulforhodamine-101, and luminescent latex particles.
    • Design and fabrication of devices for registering luminescent immunochromatograms.
    • Comparative analysis of detection sensitivity using different luminescent labels and colloidal gold conjugates.

    Main Results:

    • Luminescent submicrone latex particles demonstrated significant advantages over quantum labels, ganglioside-containing liposomes, and colloidal gold in immunochromatographic assays.
    • The developed luminescent latex-based immunochromatographic indicator element achieved a sensitivity of 3-30, outperforming elements based on colloidal gold conjugates for identical microorganisms.
    • The assay effectively detected antigens and cells of microorganisms, as well as toxins.

    Conclusions:

    • Luminescent submicrone latex particles represent a superior labeling agent for immunochromatographic assays compared to conventional labels.
    • The developed luminescent latex-based assay offers enhanced sensitivity and efficiency for detecting microbial agents and toxins.
    • This technology holds promise for improved diagnostic tools in microbiology and toxicology.

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