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Expression and regulation of IL-4 receptors on human monocytes and acute myeloblastic leukemic cells

A J Wagteveld1, A K van Zanten, M T Esselink

  • 1Department of Hematology, University of Groningen, The Netherlands.

Leukemia
|September 1, 1991
PubMed

Insights

Human monocytes and acute myeloblastic leukemic (AML) cells possess both high and low affinity interleukin-4 (IL-4) receptors. These IL-4 receptors can be modulated by external signals, and IL-4 impacts AML cell proliferation.

Area of Science:

  • Immunology
  • Cell Biology
  • Hematology

Background:

  • Interleukin-4 (IL-4) is a cytokine involved in immune responses.
  • The presence and characteristics of IL-4 receptors on human monocytes and acute myeloblastic leukemic (AML) cells are not fully understood.

Purpose of the Study:

  • To characterize the high and low affinity binding sites for IL-4 on human monocytes and AML cells.
  • To investigate the modulation of these IL-4 receptors by various stimuli.
  • To assess the functional impact of IL-4 on AML cell proliferation.

Main Methods:

  • Radioligand binding assays using radiolabeled IL-4 to quantify receptor number and affinity.
  • Cross-competition studies with other cytokines and growth factors.
  • Down-modulation studies using IL-4, protein kinase C (PKC) activators, and calcium ionophore A23187.
  • 3H-thymidine proliferation assays to assess AML cell proliferation in response to IL-4.

Main Results:

  • Both human monocytes and AML cells exhibit high and low affinity binding sites for IL-4 with comparable affinities and numbers.
  • IL-4 receptors did not show cross-competition with IL-1α, IL-3, IL-6, IL-7, G-CSF, or GM-CSF.
  • IL-4 receptors on both cell types were down-modulated by IL-4 and PKC activators, involving increased internalization and degradation.
  • IL-4 significantly affected AML cell proliferation in 8 out of 11 cases tested.

Conclusions:

  • Human monocytes and AML cells possess distinct high and low affinity IL-4 receptors.
  • These receptors can be modulated by external signals, specifically IL-4 and PKC activators.
  • IL-4 has functional implications for AML cell proliferation, suggesting a potential therapeutic target.

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