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[Effect of Ganoderma lucidum polysaccharides on intestinal mucosal immune system in H22 liver cancer bearing mice]
Gui-Qin Zhou1, Hong-Yan Zhao, Cheng Lu
1Department of Integrative Medicine, Beijing Ditan Hospital, Beijing.
Insights
Ganoderma lucidum polysaccharides (GLP) show anti-tumor effects by modulating the intestinal mucosal immune system. This study observed GLP
Area of Science:
- Immunology
- Pharmacology
- Gastroenterology
Background:
- Ganoderma lucidum polysaccharides (GLP) are known for their potential health benefits.
- The anti-tumor mechanisms of GLP are not fully understood, particularly regarding mucosal immunity.
Purpose of the Study:
- To investigate the impact of Ganoderma lucidum polysaccharides (GLP) on the mucosal immune system.
- To elucidate the anti-tumor action mechanism of GLP through its effects on intestinal immunity.
Main Methods:
- H22 tumor-bearing mice were treated with GLP, Cytoxan (CTX), or a combination.
- Immunohistochemistry and flow cytometry were used to analyze immunoglobulin A (IgA), cytokines (TNF-alpha, IL-2, IL-10), and lymphocyte subsets in the ileum.
- Analysis included intraepithelial lymphocytes (IEL), lamina propria lymphocytes (LPL), and Peyer's patch lymphocytes (PPL).
Main Results:
- Tumor-bearing mice showed significant changes in ileal lymphocyte phenotype and cytokine expression compared to controls.
- GLP treatment, alone or with CTX, modulated these immune parameters to varying degrees.
- Both GLP and CTX treatments demonstrated an ability to adjust the altered immune responses.
Conclusions:
- Ganoderma lucidum polysaccharides (GLP) exert anti-tumor effects, at least partly, through the modulation of intestinal mucosal immunity.
- GLP's influence on immune cell populations and cytokine profiles in the gut represents a potential therapeutic pathway.
Objective:
To observe the mucosal immune mechanism of anti-tumor action of Ganoderma lucidum polysaccharides (GLP).
Methods:
The concentration of H22 cells in suspension were adjusted to 1 x 10(9)/ L, and 0.2 mL of the cell suspension was injected subcutaneously in the right oxter of Kunming mice. Then the H22 bearing mice were randomly divided into 4 groups: the GLP group, the Cytoxan (CTX) group, the CTX + GLP group and the untreated model group, 8 mice in each group. Besides, a blank control group was set up. Starting from the 2nd day of modeling, GLP, at the dose of 1.02 g/kg was given to GLP group and GLP + CTX group by gastrogavage once a day for 12 successive days; CTX at the dose of 100 mg/kg was administered via peritoneal injection to the CTX group and the GLP + CTX group on the 1st day and the 6th day of the experimental course; but to the model group and the blank group, only equal volume of distilled water was given. All mice were sacrificed on the 14th day, the ileum at 1 cm upper to cecum was taken, through 4% paraform fixation and paraffin section, it was used for immunohistochemical detecting expressions of immunoglobulin A (IgA), tumor necrosis factor-alpha (TNF-alpha), interleukin-2 (IL-2) and interleukin-10 (IL-10) in ileum. Besides, the lymphocyte subsets in the intraepithelial lymphocyte (IEL), lamina propria lymphocytes (LPL), and Peyer's patch lymphocytes (PPL) were analyzed by immune fluorescence technique and flow cytometry.
Results:
Compared with the blank control group, the phenotype of lymphocytes and the expression of cytokines in ileum in the model group changed significantly; and the phenotype was variant in different regions. Compared with the model group, both indexes were adjusted in the GLP, CTX and GLP + CTX group to different degrees.
Conclusion:
The adjustment of GLP on intestinal mucosal immune is probably another path for its anti-tumor action.