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Identification of an intermediate compartment involved in protein transport from endoplasmic reticulum to Golgi

A Schweizer1, J A Fransen, K Matter

  • 1Department of Pharmacology, University of Basel, Switzerland.

European Journal of Cell Biology
|December 1, 1990
PubMed

Insights

Researchers identified a novel tubulovesicular compartment, defined by p53 protein, as a key intermediate in endoplasmic reticulum (ER)-to-Golgi protein transport. This compartment plays a crucial role in moving proteins from the ER to the Golgi apparatus.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Protein Transport

Background:

  • The endoplasmic reticulum (ER) to Golgi apparatus pathway is essential for protein modification and trafficking.
  • Understanding intermediate compartments in this pathway is crucial for deciphering cellular protein processing.

Purpose of the Study:

  • To investigate the function of a specific tubulovesicular compartment near the cis-Golgi apparatus.
  • To determine the role of this compartment in endoplasmic reticulum (ER)-to-Golgi protein transport.

Main Methods:

  • Utilized light and immunoelectron microscopy in Vero cells.
  • Studied the transport of vesicular stomatitis virus strain ts045 G protein under different temperature conditions.

Main Results:

  • Identified a 53-kDa transmembrane protein, p53, defining the tubulovesicular compartment.
  • Observed G protein accumulation in the ER at 39.5°C with access to the p53 compartment.
  • Demonstrated G protein export from the ER into the p53 compartment at 15°C, which formed a compact structure near the Golgi.
  • Showed G protein migration through the Golgi and resumption of normal p53 compartment structure at 32°C.

Conclusions:

  • Established the p53-defined compartment as the 15°C intermediate in the ER-to-Golgi protein transport pathway.
  • Highlighted the dynamic nature of this compartment during protein trafficking.

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