Related Experiment Video

Updated: Aug 12, 2026

Visualization of the Interstitial Cells of Cajal (ICC) Network in Mice
09:45

Visualization of the Interstitial Cells of Cajal (ICC) Network in Mice

Published on: July 27, 2011

Importance of ICAM-1 for accessory cell function of monocytic cells

H P Neumayer1, T F Schultz, J H Peters

  • 1Institut für Hygiene, Innsbruck, Austria.

Immunobiology
|June 1, 1990
PubMed

Insights

Intercellular Adhesion Molecule-1 (ICAM-1) is crucial for accessory cell function in certain monocyte-derived cells. However, some accessory cell functions can proceed without ICAM-1, indicating context-dependent roles.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Accessory cells are vital for T-cell activation.
  • The role of adherence molecules like ICAM-1 in monocyte-derived accessory cell function requires further elucidation.

Purpose of the Study:

  • To investigate the role of ICAM-1 in the accessory cell function of monocytes.
  • To determine the impact of ICAM-1 on T-cell stimulation by differentiated monocytes.

Main Methods:

  • Utilized monoclonal antibody 7F7 targeting ICAM-1.
  • Cultured human peripheral blood monocytes under varying conditions (serum presence/absence, duration).
  • Assessed accessory cell function and T-cell stimulation capacity.

Main Results:

  • Antibody 7F7 inhibited accessory cell function of monocytes cultured for 1-3 days.
  • Monocytes cultured for 4-7 days showed ICAM-1 expression but were not inhibited by 7F7.
  • Dendritiform accessory cells, ICAM-1 positive, had their T-cell stimulation abrogated by 7F7.

Conclusions:

  • ICAM-1 contributes to T-cell stimulation by accessory cells at specific differentiation stages.
  • Accessory cell function can occur independently of ICAM-1 in certain contexts, suggesting alternative interaction pathways.

More Related Videos

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions07:40

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

Published on: June 29, 2016

Artificial Intelligence Approaches to Assessing Primary Cilia08:58

Artificial Intelligence Approaches to Assessing Primary Cilia

Published on: May 1, 2021

Related Concept Videos

Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily  involved in a...
Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily  involved in a...