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Proliferation index in tuberculous lymphadenitis

Pukpinya Jangjetriew1, Sanya Sukpanichnant

  • 1Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. Drbanchong_jang@yahoo.com

Asian Pacific Journal of Allergy and Immunology
|October 21, 2009
PubMed

Insights

Activated macrophages (AMs) in granulomas can proliferate, as indicated by mitotic figures and a proliferation index (PI). This study evaluated AM proliferation in tuberculous lymphadenitis biopsies.

Area of Science:

  • Immunology
  • Pathology
  • Cell Biology

Background:

  • Granuloma formation involves activated macrophages (AMs) recruited by migration inhibition factor.
  • The self-proliferation capacity of AMs within granulomas remains an area of investigation.

Purpose of the Study:

  • To investigate the proliferative capacity of activated macrophages (AMs) within granulomas.
  • To quantify AM proliferation in tuberculous lymphadenitis using the proliferation index (PI).

Main Methods:

  • Immunohistochemistry utilizing the MIB-1 antibody against Ki-67 was performed on lymph node biopsies from 40 tuberculous lymphadenitis cases.
  • The proliferation index (PI) was calculated as the percentage of AMs with positive nuclear staining.
  • Mitotic figures, multinucleated giant cells, and caseous necrosis were also assessed.

Main Results:

  • The proliferation index (PI) of AMs ranged from 3.6% to 20.6% (mean 11.0 +/- 4.4%).
  • Mitotic figures were observed (0-6 per 500 AMs), and multinucleated giant cells had a PI of 0%.
  • No statistically significant correlation was found between PI and mitotic figures, multinucleated giant cells, or caseous necrosis.

Conclusions:

  • Activated macrophages (AMs) within granulomas demonstrate a limited capacity for self-proliferation.
  • The proliferation index and mitotic figures are indicators of AM proliferation in the context of granuloma pathogenesis.

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