Related Experiment Video

Updated: Jun 17, 2026

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
11:53

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Published on: July 1, 2014

Evaluation of isotope-coded protein labeling (ICPL) in the quantitative analysis of complex proteomes

Alberto Paradela1, Miguel Marcilla, Rosana Navajas

  • 1Laboratorio de Proteómica, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain. alberto.paradela@cnb.csic.es

Talanta
|January 20, 2010
PubMed

Insights

Isotope-coded protein labeling (ICPL) is a viable technique for differential quantitative proteomics. Optimizing ICPL workflow and labeling at the peptide level can enhance protein quantification and reliability.

Area of Science:

  • Proteomics
  • Quantitative Biology
  • Biotechnology

Background:

  • Differential quantitative proteomics enables the comparison of protein expression levels between samples.
  • Isotope-coded protein labeling (ICPL) is a non-isobaric labeling technique for quantitative proteomics.

Purpose of the Study:

  • To evaluate the ICPL technique using two biological models.
  • To optimize ICPL workflow parameters.
  • To compare ICPL with traditional 2D-PAGE methods.

Main Methods:

  • Phage T4 capsids labeled with light/heavy ICPL reagents and mixed in a 1:1 ratio.
  • Analysis of Salmonella enterica serovar Typhimurium virulent and attenuated strains using ICPL.
  • Comparison of ICPL results with 2D-PAGE.

Main Results:

  • ICPL demonstrated feasibility for differential quantitative proteomics.
  • Experimental parameters for ICPL workflow were optimized.
  • ICPL proved a valuable alternative to other labeling techniques, including 2D-PAGE.
  • Labeling at the peptide level suggested increased protein quantification and reliability.

Conclusions:

  • ICPL is a robust technique for quantitative proteomics.
  • Further improvements in ICPL can be achieved by labeling at the peptide level.
  • ICPL offers a valuable alternative for differential protein expression analysis.

More Related Videos

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)09:06

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)

Published on: May 1, 2017

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Related Concept Videos

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...