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[Cytoplasmic immunoglobulins in chronic B-lymphoid leukemia]
J F Filippi1, C Nicolino-Brunet, N Tubiana
1Laboratoire d'Hématologie Biologique, Faculté de Médecine et de Pharmacie, Marseille, France.
Insights
Detecting both surface and cytoplasmic immunoglobulins in chronic lymphocytic leukemia B-cells significantly improves monoclonality assessment. This combined approach is more effective than surface immunoglobulin analysis alone for diagnosing B-cell chronic lymphocytic leukemia.
Area of Science:
- Hematology
- Immunology
- Oncology
Context:
- Chronic lymphocytic leukemia (CLL) is a common B-cell malignancy.
- Assessing monoclonality is crucial for CLL diagnosis and management.
- Surface immunoglobulin (Smlg) and cytoplasmic immunoglobulins (Clg) are key B-cell markers.
Purpose:
- To evaluate the diagnostic utility of combined surface and cytoplasmic immunoglobulin detection in B-cell chronic lymphocytic leukemia (B-CLL).
- To assess the expression of CD10 and other membrane markers in B-CLL.
- To explore correlations between immunologic markers and clinical staging in B-CLL.
Summary:
- A study of 60 B-CLL cases found that combined Smlg and Clg determinations identified monoclonality in 65% of cases, compared to 38% with Smlg alone.
- Monoclonal antibody analysis revealed CD10 positivity in 36% of patients.
- No significant correlations were observed between immunologic markers and clinical stage.
Impact:
- Combined immunoglobulin analysis enhances the diagnostic accuracy for B-cell chronic lymphocytic leukemia.
- This improved diagnostic approach aids in better patient stratification and treatment planning.
- Understanding B-cell marker expression provides insights into CLL pathogenesis and potential therapeutic targets.
Abstract:
Chronic lymphocytic leukemia B-cells (B-CLL cells) have surface immunoglobulin (Smlg). In addition, cytoplasmic immunoglobulins (Clg) have been reported in chronic lymphocytic leukemia B-cells and are useful for ascertaining monoclonality of the disease. In a study of 60 cases of chronic lymphocytic leukemia B-cells, surface immunoglobulin determinations alone demonstrated monoclonality in 38% of cases, versus 65% of cases with combined surface immunoglobulin and cytoplasmic immunoglobulins determinations. Membrane markers were studied using a panel of monoclonal antibodies. Thirty-six per cent of patients were positive for CD 10. No correlations were seen between immunologic markers and clinical stage.