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Updated: Jun 15, 2026
Mouse Wound Models and Preparation of Single-Cell Suspensions
Published on: September 27, 2024
[Expression of c-jun during the incised wound healing in mice skin]
Me Yang1, Da-Wei Guan, Chang-Yan Xiong
1School of Forensic Medicine, China Medical University, Shenyang 110001, China. ymdoctor@163.com
Insights
The expression of c-jun, a key protein, changes over time during mouse skin wound healing. This protein
Area of Science:
- Cellular biology
- Molecular biology
- Dermatology
Context:
- Skin wound healing is a complex biological process.
- Understanding the molecular mechanisms is crucial for therapeutic interventions.
- The role of specific proteins like c-jun in this process requires detailed investigation.
Purpose:
- To investigate the temporal expression patterns of c-jun during the healing of incised wounds in mouse skin.
- To correlate c-jun expression levels with distinct phases of wound repair.
Summary:
- c-jun expression was low in normal skin but increased significantly after injury, peaking at 12 hours and 7 days.
- Immunohistochemistry revealed c-jun in neutrophils, mononuclear cells (MNCs), and fibroblasts at different time points.
- Western blot analysis confirmed two major expression peaks, indicating dynamic regulation during healing.
Impact:
- c-jun may induce apoptosis in inflammatory and reparative cells during skin healing.
- The distinct temporal expression of c-jun suggests its potential utility as a biomarker for determining wound age.
- Findings contribute to a deeper understanding of molecular events in skin regeneration.
Unlabelled:
OBJECTIVE To investigate the time-dependent expression of c-jun during the healing of incised wound in mice skin.
Methods:
The expression of c-jun in different stages after the incised wound were detected by immunohistochemistry and Western blot.
Results:
There was a low level expression of c-jun in normal mice skin. Expression of c-jun was mainly detected in neutrophils from 3 h to 12h after injury. The c-jun positive cells were almost mononuclear cells (MNCs) and fibroblasts between 1 d and 5 d after injury. The c-jun positive cells were mostly fibroblasts between 7 d and 14 d after injury. The ratio of the c-jun positive cells increased in the wound specimens from 3 h to 12 h, peaked at 12 h, declined partially from 1 d to 5 d, and reached the peak secondly at 7 d, then decreased from 10 d to 14 d. The expression of c-jun was observed throughout the wound healing stages by Western blot with two peaks occurring at 12 h and 7 d after injury.
Conclusion:
The c-jun may play a potential role in inducing apoptosis of neutrophils, MNCs and fibroblasts during skin wound healing, and it may be used as the marker for wound age determination.
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