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A monoclonal antibody to human leukocyte common antigen, SHL-1, and its use for formalin-fixed, paraffin-embedded
1Department of Pathology, College of Medicine, Seoul National University, Korea.
Insights
A new monoclonal antibody, SHL-1, targets human leukocyte common antigen (LCA) family members. This antibody effectively identifies most leukocytes and hematopoietic malignancies, aiding in diagnosing hematological disorders.
Area of Science:
- Immunology
- Hematology
- Oncology
Background:
- A novel murine monoclonal antibody, SHL-1, has been characterized.
- SHL-1 targets a human leukocyte surface antigen belonging to the leukocyte common antigen (LCA) family.
- The SHL-1 antigen has a molecular weight of approximately 180,000 daltons and is resistant to standard tissue fixation.
Purpose of the Study:
- To evaluate the utility of the SHL-1 antibody in immunohistochemical staining of paraffin-embedded tissues.
- To assess the reactivity of SHL-1 with various hematopoietic and non-hematopoietic malignancies.
- To determine the diagnostic and research applications of SHL-1 in hematological disorders.
Main Methods:
- Indirect immunoperoxidase technique for screening fresh and paraffin-embedded tissues.
- Immunofluorescence method for determining immunoreactivity in leukemic samples and tumor cell lines.
- Wide screening across a diverse range of normal leukocytes and various malignancies.
Main Results:
- SHL-1 labeled the majority of normal leukocytes and hematopoietic malignancies.
- Non-hematologic malignancies were completely negative for SHL-1.
- 23 out of 24 leukemic preparations showed strong reactivity, with one B-cell leukemia case being non-reactive.
- SHL-1 demonstrated higher reactivity towards T-cell series than B-cell series.
Conclusions:
- The SHL-1 antibody is valuable for immunohistochemical staining of paraffin-embedded tissues.
- SHL-1 is a useful diagnostic and research tool for hematological disorders, particularly effective in identifying T-cell related antigens.
- Its specificity for hematopoietic cells aids in differentiating lymphomas and leukemias from non-hematologic malignancies.
Abstract:
The authors describe a newly characterized murine monoclonal antibody to the human leukocyte surface antigen, SHL-1. The antigen belongs to the leukocyte common antigen (LCA) family, and its molecular weight is about 180,000 daltons, which is similar to that of some previously characterized LCAs. The SHL-1 antigen is resistant to conventional tissue-fixation and embedding procedures. This antibody can therefore be used in the immunohistochemical staining of paraffin-embedded tissue sections. Wide screening with a sufficient number of both fresh and routinely processed paraffin-embedded tissues was done with indirect immunoperoxidase technique. With this procedure, SHL-1 labeled the majority of normal leukocytes and hematopoietic malignancies. Some B-cell malignancies were not stained with this antibody. The non-hematologic malignancies posing diagnostic problems of differentiation from lymphomas or leukemias were completely negative to SHL-1. The immunoreactivity to SHL-1 of samples from 24 leukemic patients and 15 human tumor cell lines was determined by the immunofluorescence method. Of 24 leukemic preparations, 23 were strongly reactive to this antibody. One case of B-cell leukemia did not react with SHL-1. No immunoreactivity was demonstrated in non-hematopoietic tumor cell lines. The overall reaction pattern of SHL-1 proved its usefulness in both diagnostic and research practice in hematological disorders. This antibody detected cell surface antigens of the T cell series more effectively than those of the B-cell series in terms of the positive number of cells and mean fluorescence intensity.
