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Human Th1 cells that express CD300a are polyfunctional and after stimulation up-regulate the T-box transcription

Sriram Narayanan1, Rodolfo Silva, Giovanna Peruzzi

  • 1Receptor Cell Biology Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, United States of America.

Plos One
|May 26, 2010
PubMed

Insights

CD300a expression identifies a subset of human effector CD4 T cells that are polyfunctional and produce Th1 cytokines. These cells up-regulate eomesodermin (Eomes) upon stimulation, a process influenced by TGF-beta1.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Naïve CD4 T cells express low CD300a, while effector/memory cells show variable expression.
  • CD300a expression may define a distinct subset within effector/memory CD4 T cells.

Purpose of the Study:

  • To investigate the functional characteristics of CD300a-expressing CD4 T cells.
  • To determine the role of CD300a in defining Th1 cell subsets and their associated transcription factors.

Main Methods:

  • Ex vivo analysis of human CD4 T cells.
  • Flow cytometry to assess CD300a expression.
  • Measurement of cytokine production (IFN-gamma, TNF-alpha, IL-2) and transcription factor expression (Eomes, T-bet).

Main Results:

  • CD4 T cells producing IFN-gamma are enriched in the CD300a(+) subset.
  • Polyfunctional CD4 T cells (producing IFN-gamma, TNF-alpha, IL-2) are predominantly CD300a(+).
  • Stimulated CD300a(+) CD4 T cells exhibit increased Eomes expression, while T-bet is similarly upregulated in both CD300a(+) and CD300a(-) cells.

Conclusions:

  • CD300a(+) human Th1 cells are often polyfunctional and up-regulate Eomes after stimulation.
  • TGF-beta1 inhibits CD300a expression and down-regulates Eomes and IFN-gamma, suggesting a role in dictating Th1 subset development.

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