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Malignant lymphoma in pleural effusions: an immunocytochemical cell surface analysis

J Guzman1, K J Bross, U Costabel

  • 1Pathologisches Institut der Universitaet, Freiburg, Federal Republic of Germany.

Diagnostic Cytopathology
|January 1, 1991
PubMed

Insights

Immunocytochemical analysis of pleural effusions aids in identifying lymphoma cells, including B-cell non-Hodgkin

Area of Science:

  • Hematology
  • Oncology
  • Immunology

Background:

  • Malignant pleural effusions can be challenging to diagnose.
  • Lymphoma subtypes present unique diagnostic profiles.

Purpose of the Study:

  • To evaluate the utility of immunocytochemistry in diagnosing lymphoma in malignant pleural effusions.
  • To characterize cell surface antigen expression in various lymphoma subtypes within pleural effusions.

Main Methods:

  • Analysis of nine malignant pleural effusions using peroxidase-antiperoxidase immunocytochemistry.
  • Utilized a broad panel of monoclonal antibodies to detect cell surface antigens.
  • Examined lymphoma subtypes including hairy-cell leukemia, B-cell non-Hodgkin's lymphoma, Hodgkin's disease, and plasmacytoma.

Main Results:

  • B-cell lymphomas showed high percentages of kappa-positive B cells.
  • Hairy cells reacted with CD20, CD25, HLA-DR, CD45, and HLA-1.
  • Hodgkin cells reacted with CD15, CD20, CD25, CD30, Tü9, and OKT9.
  • Plasmacytoma cells exhibited specific antigen profiles including lambda positivity.

Conclusions:

  • Immunocytochemistry effectively identifies B lymphoma, Hodgkin, and hairy cells in pleural effusions.
  • Non-malignant lymphocyte subsets in these effusions are similar to other effusions.
  • This technique offers clear recognition of malignant cells in pleural fluid.

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