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Published on: November 10, 2016
An amperometric immunosensor based on a polyelectrolyte/gold magnetic nanoparticle supramolecular assembly--modified
Ning Gan1, Jianguo Hou, Futao Hu
1The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Material Science and Chemical Engineering, Ninbo University, Ningbo, 315211, China. ganning@nbu.edu.cn
Insights
A novel immunosensor detects Human Immunodeficiency Virus antigen p24 (HIV p24) using self-assembly. This sensitive biosensor offers a promising tool for early AIDS diagnosis.
Area of Science:
- Biosensors and Nanotechnology
- Analytical Chemistry
- Immunodiagnostics
Background:
- Early diagnosis of Human Immunodeficiency Virus (HIV) is crucial for effective management.
- Detection of HIV p24 antigen is a key biomarker for early HIV infection.
- Development of sensitive and reliable immunosensors is essential for clinical diagnostics.
Purpose of the Study:
- To develop a novel supramolecular amperometric immunosensor for the sensitive determination of HIV p24 antigen.
- To utilize electrostatic layer-by-layer self-assembly for fabricating the immunosensor on a gold electrode.
- To evaluate the performance and applicability of the developed immunosensor for HIV p24 detection.
Main Methods:
- Fabrication of a gold electrode modified with polyelectrolyte/gold nanoparticle multilayer films.
- Immobilization of anti-HIV p24 antibodies onto the multilayer films.
- Amperometric detection of HIV p24 antigen based on the inhibition of electron transfer.
- Characterization of the immunosensor using X-ray fluorescence spectrometry, SEM, and TEM.
Main Results:
- The immunosensor demonstrated high sensitivity for p24 detection, with a linear range of 0.1–100.0 ng/mL.
- A low detection limit of 0.05 ng/mL was achieved at a signal-to-noise ratio of 3.
- The immunosensor showed good correlation when analyzing p24 in human serum specimens.
- The electron transfer of FeCN was partially inhibited upon incubation with HIV p24, leading to a decreased peak current.
Conclusions:
- The developed supramolecular amperometric immunosensor is a sensitive and reliable tool for HIV p24 detection.
- This novel biosensor offers a promising alternative for the early diagnosis of AIDS patients.
- The layer-by-layer self-assembly technique provides an effective platform for immunosensor fabrication.
Abstract:
A novel supramolecular amperometric immunosensor for the determination of Human Immunodeficiency Virus antigen p24 (HIV p24) was built up using the electrostatic layer-by-layer self-assembly technique upon a gold electrode with HIV p24 antibody (anti-p24) being immobilized on polyelectrolyte/gold nanoparticle multilayer films. The multilayer films were composed of poly(L-lysine) (pLys) and mercaptosuccinic acid (MSA) stabilized Fe(3)O(4)(core)/gold(shell) nano particles (GMPs).The immunosensor preparation steps were monitored by X-ray fluorescence spectrometry (XRFS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). In pH 6.5 PBS, after the immunosensor was incubated with HIV p24 solution at 25 degrees C for 5 min, the electron transfer access of FeCN is partially inhibited, which leads to a linear decrease of peak current. In addition, the performance of the immunosensor was studied in detail. It offers high-sensitivity for the detection of p24 and has good correlation for the detection of p24 in the range of 0.1 to 100.0 ng/mL with a detection limit of 0.05 ng/mL estimated at a signal-to-noise ratio of 3. The proposed immunosensor was used to analyze p24 in human serum specimens and the results showed the developed immunosensor provides a promising alternative approach for detecting p24 in the early diagnosis of AIDS patients.
