Related Experiment Video
Updated: Jun 10, 2026
Assessment of the Synaptic Interface of Primary Human T Cells from Peripheral Blood and Lymphoid Tissue
Published on: July 30, 2018
Evaluation of the effect of IL-22 on human cord blood CD4+ T cells
Javad Arasteh1, Zahra Pourpak, Masoumeh Ebtekar
1Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
Insights
Interleukin-22 (IL-22) does not influence the expression of CD25 or Foxp3 in cord blood T cells. This study found IL-22 does not affect T cell proliferation or suppression, suggesting a lack of IL-22 receptor 1 on these cells.
Area of Science:
- Immunology
- Cell Biology
Background:
- Interleukin-22 (IL-22) is a cytokine in the IL-10 family, implicated in inflammatory responses.
- IL-22 shares similarities with IL-10, including conserved sequences and receptor components (IL-22R1 and IL-10R2).
Purpose of the Study:
- To investigate the biological activity of IL-22 on human cord blood CD4+CD25- T cells.
- To determine IL-22's effect on T cell activation, proliferation, and regulatory function.
Main Methods:
- Cord blood CD4+CD25- T cells were cultured with anti-CD3/CD28 beads, IL-2, and IL-22 for two weeks.
- Flow cytometry was used to analyze CD25 and Foxp3 expression.
- T cell proliferation was assessed, and suppression assays were performed.
Main Results:
- IL-22 did not significantly alter CD25 or Foxp3 expression in cultured T cells.
- IL-22 was found to have no impact on CD4+ T cell proliferation.
- No suppressive effect of IL-22 was observed in the conducted assays.
Conclusions:
- Umbilical cord blood T cells likely do not express the IL-22 receptor 1 (IL-22R1) on their surface.
- IL-22 does not appear to play a direct role in the early activation or function of these specific T cells.
Abstract:
IL-22 is a member of IL-10 cytokine family which is believed to play an important role in inflammatory responses. IL-22 has similarities with IL-10 including conserved sequences with IL-10. IL-22 receptor is also comprised of two chains known as L-22R1 and L-10R2; supporting the speculation that the two cytokines may have similar effects. The aim of this study was to shed some light on the biological activity of IL-22 upon the cord blood CD4+CD25- T cells. In this research, cord blood T CD4+CD25- cells were cultured in presence of anti CD2/CD3/CD28 coated beads, IL-2 and IL-22 for two weeks at 37 degrees C and 5% CO2. Flow cytometry analysis showed that IL-22 has no effect upon CD25 and Foxp3 expression. Also, the results indicated that IL-22 is not involved in CD4+ T cell proliferation. Moreover, the results of suppression assay did not show any suppression effect on the cultured T cells. Thus, it seems that umbilical cord blood T cells probably do not express IL-22R1 on their surface.
