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Karyopyknotic cytoplasmic inclusions in neutrophils

Menchu C Ong1, Diana M Veillon, Mary Lowery Nordberg

  • 1Louisiana State University Health Sciences Center Department of Pathology, Shreveport, USA.

The Journal of the Louisiana State Medical Society : Official Organ of the Louisiana State Medical Society
|October 2, 2010
PubMed

Insights

Karyopyknotic cytoplasmic inclusions in neutrophils (KPCI), previously termed "Howell-Jolly" like, are DNA-based. This study confirms their deoxyribonucleic acid origin and describes new forms in neutrophils.

Area of Science:

  • Hematology
  • Infectious Diseases
  • Oncology

Background:

  • Karyopyknotic cytoplasmic inclusions in neutrophils (KPCI) are observed in immunosuppressed patients, including those with human immunodeficiency virus (HIV), and in patients with malignancies undergoing chemotherapy.
  • These inclusions have been historically referred to as "Howell-Jolly" like inclusions.
  • Previous characterization attempts using stains like Grocott's methenamine silver (GMS), periodic acid Schiff (PAS), Gram, and Feulgen suggested a deoxyribonucleic acid (DNA) origin.

Observation:

  • This case report details an additional method to confirm the deoxyribonucleic acid (DNA) nature of KPCI.
  • The study documents previously undescribed curvilinear forms of these inclusions.

Findings:

  • The findings confirm that KPCI are of deoxyribonucleic acid (DNA) origin.
  • Novel curvilinear morphologies of KPCI have been identified.

Implications:

  • This research provides further evidence for the composition of KPCI, aiding in their accurate identification.
  • The documentation of new forms may enhance diagnostic capabilities for conditions associated with KPCI.
  • Understanding KPCI composition and morphology can contribute to the study of neutrophil pathology in immunocompromised states.

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