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Updated: Jun 7, 2026
Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
Published on: April 16, 2016
Cryoglobulin detection from blood and peritoneal fluid smears
J-F Lesesve1, M Muller, A Vautrin
1Service d'Hématologie Biologique, Centre Hospitalier Universitaire, Nancy, France. jf.lesesve@chu-nancy.fr
Insights
Cytoplasmic inclusions in white blood cells (WBCs) can indicate cryoglobulinemia. This study highlights cryoglobulins specifically in neutrophils and monocytes, and their detection in body fluids.
Area of Science:
- Hematology
- Clinical Pathology
- Immunology
Background:
- Cryoglobulinemia is a condition characterized by abnormal proteins in the blood that precipitate in cold temperatures.
- Previous studies have reported cytoplasmic inclusions in leukocytes as a rare manifestation of cryoglobulinemia.
Observation:
- This study presents two novel observations regarding cryoglobulinemia.
- The first observation details a specific pattern of cryoglobulin deposition within neutrophils and monocytes, notably excluding other white blood cell types.
- The second observation emphasizes the potential for identifying these cryoglobulin deposits morphologically in various body fluid samples.
Findings:
- Cryoglobulins exhibited a distinct localization pattern, primarily affecting neutrophils and monocytes.
- Morphological identification of cryoglobulin deposits in body fluids is feasible.
- This specific cellular tropism and detectability offer new diagnostic insights.
Implications:
- These findings enhance the understanding of cryoglobulinemia's diverse clinical presentations.
- The specific identification in neutrophils and monocytes may refine diagnostic criteria.
- Morphological analysis in body fluids could provide a non-invasive diagnostic approach.
Abstract:
Laboratory identification of cytoplasmic inclusions in leucocytes as unusual manifestation of cryoglobulinemia has been previously reported (Maitra et al., 2000 American Journal of Clinical Pathology, 113, 107-112; Fohlen-Walter et al., 2002 American Journal of Clinical Pathology, 117, 606-614.). We would like to add two observations highlighting the following: (i) the peculiar picture of cryoglobulins in neutrophils and monocytes but sparing other white blood cell (WBC) and (ii) possibility of deposit occurrence with morphological identification in body fluids.
