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Updated: Aug 8, 2026
Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
Published on: November 14, 2016
[Histological study of the lymph nodes of cattle, before and after antigenic stimulation. 2. Immunocytochemistry and
F Femenia1, T Alogninouwa, J Perre
1Laboratoire d'Histologie et Anatomie Pathologique, INRA, Ecole Nationale Vétérinaire, Maisons Alfort, France.
Insights
This study used advanced microscopy and staining to identify various cells in bovine lymph nodes. These methods are effective for examining normal and diseased lymph nodes in cattle.
Area of Science:
- Veterinary Pathology
- Immunohistochemistry
- Electron Microscopy
Context:
- Bovine lymph nodes are crucial for immune responses.
- Understanding cellular composition aids in diagnosing diseases.
- Previous studies lacked detailed cellular characterization.
Purpose:
- To characterize cellular populations in stimulated and non-stimulated bovine lymph nodes.
- To evaluate the utility of immunocytochemistry and electron microscopy for bovine lymph node analysis.
- To identify lymphoid and non-lymphoid cells, including antigen-presenting cells and T lymphocytes.
Summary:
- Immunohistochemistry identified cytoplasmic immunoglobulins in lymphoid cells and S100 protein in antigen-presenting cells, macrophages, and endothelial cells.
- Monoclonal antibodies (M1, M12) were used to identify T lymphocytes.
- Electron microscopy confirmed the localization and identification of various cell types within the lymph node structure.
Impact:
- The study validates immunocytochemistry and electron microscopy as suitable techniques for bovine lymph node research.
- Findings provide a foundation for further investigations into bovine lymph node function and pathology.
- This research supports improved diagnostic capabilities for bovine lymph node diseases.
Abstract:
A histological and cytological study of stimulated and non-stimulated bovine lymph nodes was performed using immunocytochemistry and electron-microscopic techniques. Immunohistochemical staining using polyclonal antibodies revealed cytoplasmic immunoglobulins producing lymphoid cells. A protein S100 specific serum evidenced antigen presenting cells, macrophages and endothelial cells. In order to identify T lymphocytes, two monoclonal antibodies (M1 and M12) were used. The electron-microscopic study confirmed the localization and identification of different lymphoid and non-lymphoid cells of the lymph node. The techniques used in the present work seem to be suitable for investigating bovine lymph nodes and those with pathologic alterations.
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