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Dual amplification strategy for the fabrication of highly sensitive interleukin-6 amperometric immunosensor based on
Guangfeng Wang1, Hao Huang, Ge Zhang
1Key Laboratory of Chem-Biosensing, Anhui Province, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
Insights
A novel electrochemical immunosensor offers sensitive detection of Interleukin-6 (IL-6). This dual amplification system uses Au nanoparticles-Poly-dopamine and enzyme-antibody functionalized nanomaterials for enhanced performance.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Analytical Chemistry
Background:
- Interleukin-6 (IL-6) is a key biomarker in inflammatory diseases.
- Sensitive and selective detection of IL-6 is crucial for early diagnosis and monitoring.
- Existing detection methods may lack sensitivity or require complex procedures.
Purpose of the Study:
- To develop a highly sensitive electrochemical immunosensor for IL-6 detection.
- To utilize a dual amplification strategy for enhanced signal generation.
- To validate the sensor's performance in complex biological samples like human serum.
Main Methods:
- Fabrication of a sensor platform using gold nanoparticles (AuNP) and Poly-dopamine (PDOP).
- Immobilization of biomolecules on the AuNP-PDOP platform for sensor construction and signal labeling.
- Synthesis of multienzyme-antibody functionalized AuNP-PDOP@carbon nanotubes (CNT) for signal amplification.
- Amperometric determination for measuring IL-6 concentration.
Main Results:
- Achieved a linear response range for IL-6 from 4.0 to 8.0 × 10^2 pg mL⁻¹.
- Obtained a low detection limit of 1.0 pg mL⁻¹ for IL-6.
- Demonstrated excellent correlation with standard ELISA assays in human serum samples.
- Exhibited high selectivity, good reproducibility, and stability of the immunosensor.
Conclusions:
- The developed electrochemical immunosensor provides sensitive and reliable detection of IL-6.
- The dual amplification mechanism significantly enhances detection performance.
- The sensor shows potential for clinical applications in diagnosing IL-6 related conditions.
Abstract:
An electrochemical immunosensor was studied for sensitive detection of Interleukin-6 (IL-6) based on a dual amplification mechanism resulting from Au nanoparticles (AuNP)-Poly-dopamine (PDOP) as the sensor platform and multienzyme-antibody functionalized AuNP-PDOP@carbon nanotubes (CNT). The stable and robust film, PDOP, was used to immobilize biomolecules not only for the construction of the sensor platform, but also for the signal labeling. Sensitivity was greatly amplified by using the special platform of AuNP-PDOP and synthesizing horseradish peroxidase (HRP)-antibody (Ab(2)) functionalized AuNP-PDOP@carbon nanotubes (CNT). A linear response range of IL-6 from 4.0 to 8.0 × 10(2) pg mL(-1) with a low detection limit of 1.0 pg mL(-1) was obtained by the amperometry determination. Measurements of IL-6 in human serum gave excellent correlations with standard ELISA assays. Moreover, the immunosensor exhibited high selectivity, good reproducibility, and stability.
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