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Cytopathology of canine lymphomas (100 cases)
R Sapierzyński1, J Micuń, D Jagielski
1Department of Pathology and Veterinary Diagnostic, Faculty of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159c, 02-766 Warsaw, Poland. rafal-sapierzynski@sggw.pl
Insights
Malignant lymphoma is a common canine tumor. This study analyzed 100 dogs using fine needle aspiration biopsy, finding high-grade lymphomas were most prevalent, especially in mixed breeds.
Area of Science:
- Veterinary Pathology
- Canine Oncology
- Cytopathology
Background:
- Malignant lymphoma is a frequent cancer in dogs.
- Fine needle aspiration biopsy (FNAB) is a key diagnostic tool for canine lymph node assessment.
Purpose of the Study:
- To perform a cytopathological analysis of canine lymphoma.
- To investigate epidemiological aspects of lymphoma occurrence in 100 dogs.
Main Methods:
- Cytopathological analysis of lymphoma using Giemsa-stained slides.
- Samples collected via fine needle aspiration biopsy, non-aspiration biopsy, lymph node impression smears, and body cavity effusions.
- Tumor type and subtype determined using the updated Kiel classification for dogs.
Main Results:
- Lymphoma diagnosed in 100 dogs (44 female, 56 male) aged 1.5-15 years.
- High-grade lymphomas (86%) were more common than low-grade (14%).
- Boxer dogs may have a predisposition to T-cell lymphoma.
Conclusions:
- Cytopathology is effective for diagnosing canine lymphoma.
- High-grade lymphomas are predominant in the studied canine population.
- Further research is warranted regarding breed predispositions, such as Boxers and T-cell lymphoma.
Abstract:
Malignant lymphoma is one of the most common malignant tumours occurring in dogs. Fine needle aspiration biopsy (FNAB) is an excellent, specific diagnostic procedure used to assess pathological processes in lymph nodes. The aim of the present study was to conduct a cytopathological analysis of lymphoma in dogs and to analyse some epidemic aspects of occurrence of lymphoma in 100 dogs using Giemsa stained slides. Samples were obtained by fine needle aspiration biopsy, fine needle non-aspiration biopsy, lymph node impression smears and by examination of body cavity effusions. The determination of the type and subtype of tumour was made on the basis of the updated Kiel classification adopted for dogs. Based on cytopathological analysis, the lymphoma was diagnosed in 100 dogs: 44 were female and 56 male. The animals' age ranged from 1.5 year to 15 years (median: 7.5 years), the animals were of different breeds (72% of all dogs belonged to 28 different breeds) and crossbreeds (28%). In 29% of dogs the regional or general lymphadenomegaly was the only clinical sign observed, in remaining cases (71%) at least one abnormality connected to lymphoma was found. Among all diagnosed lymphomas, high-grade lymphomas were more prevalent (86% of all cases) than low-grade lymphomas (14% of all cases). The possibility of boxers having a predisposition to T cell lymphoma development could be also suspected.
