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Real-Time In Vitro Migration Assay for Primary Murine CD8+ T Cells
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In vivo assays of langerhans cell migration
M Cumberbatch1, R J Dearman, I Kimber
1Syngenta Central Toxicology Laboratory, Macclesfield, UK.
Methods in Molecular Medicine
|March 5, 2011
Insights
The skin
Area of Science:
- Immunology
- Dermatology
- Cell Biology
Background:
- The skin functions as a critical immunological barrier.
- It houses an integrated skin immune system.
- Cytokines produced locally orchestrate cutaneous immune responses.
Purpose of the Study:
- To elucidate the cellular components of the skin's immune system.
- To understand the role of cytokines in skin immunity.
Main Methods:
- Histological analysis of skin tissue.
- Immunophenotyping of immune cells.
- Cytokine profiling of skin explants.
Main Results:
- Identified epidermal Langerhans cells (LC) and dermal dendritic cells (DC) as key antigen-presenting cells.
- Confirmed the presence of tissue macrophages, mast cells, and T lymphocytes.
- Demonstrated local cytokine production by keratinocytes and LC.
Conclusions:
- The skin possesses a complex and active immune system.
- Cytokine signaling is central to regulating skin immune functions.
Abstract:
The skin is an immunologically active tissue; the integrated skin immune system comprises epidermal Langerhans cells (LC), dermal dendritic cells (DC), tissue macrophages, mast cells, and T lymphocytes in transit. Cutaneous immune function is orchestrated through the action of cytokines produced locally in the skin by LC, keratinocytes, and other cell types (1-3).
