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Generation of Human CD40-activated B cells
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Characterization of the common acute lymphoblastic leukaemia antigen (CD10) as an activation molecule on mature human

N Kiyokawa1, Y Kokai, K Ishimoto

  • 1Department of Pathology, National Children's Medical Research Centre, Tokyo, Japan.

Clinical and Experimental Immunology
|March 1, 1990
PubMed

Insights

CD10 is transiently expressed early in B cell activation, distinct from other activation markers. This pattern mirrors Burkitt

Area of Science:

  • Immunology
  • Cell Biology
  • Hematology

Background:

  • CD10 is a cell surface marker with a known role in B cell development.
  • The precise expression pattern of CD10 during B cell activation requires further elucidation.
  • Understanding B cell activation markers is crucial for diagnosing and classifying lymphoid malignancies.

Purpose of the Study:

  • To analyze the distinct expression pattern of CD10 during B cell activation.
  • To investigate the relationship between CD10 expression and other activation markers.
  • To explore the potential role of CD10 expression in the histogenesis of Burkitt's lymphoma.

Main Methods:

  • Two-color flow cytometry was used to analyze CD10 expression on activated B cells.
  • In vivo and in vitro systems were employed to study B cell activation.
  • Cell fractionation by gravity was performed to separate cell populations based on antigen expression.
  • Immunophenotyping of Burkitt's lymphoma cases was conducted.

Main Results:

  • CD10 is expressed at a specific, transient early stage of B cell activation, distinct from other activation antigens.
  • CD10+ B cells could be separated from cells expressing other activation markers and some CD10+ cells also expressed the resting B cell antigen L30.
  • In vitro, CD10+ B cells emerged early and disappeared late during activation.
  • All analyzed Burkitt's lymphoma cases expressed CD10, with a high co-expression of L30 and L29.

Conclusions:

  • CD10 is a transiently expressed early activation antigen on B cells.
  • The immunophenotype of Burkitt's lymphoma suggests a resemblance to early-stage activated B cells co-expressing CD10 and L30.
  • This study provides insights into the precise expression dynamics of CD10 and its implications for understanding Burkitt's lymphoma.

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