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Immunohistochemical localization of a plasma protein (glycoprotein 60) which inhibits complement-mediated prevention

G P Sandilands1, A E Ahmed, M R Griffiths

  • 1University Department of Pathology, Western Infirmary, Glasgow.

Immunology
|July 1, 1990
PubMed

Insights

Glycoprotein 60 (gp60), an IgG Fc-binding protein, is located in human hepatocytes, platelets, and lymphocytes. This protein is distinct from known Fc gamma receptors.

Area of Science:

  • Immunology
  • Biochemistry

Background:

  • Glycoprotein 60 (gp60) is an IgG Fc-binding protein.
  • It was recently isolated from normal human plasma.

Purpose of the Study:

  • To determine the localization of gp60 in human tissues and cells.
  • To compare gp60 with previously identified IgG-binding proteins and Fc gamma receptors.

Main Methods:

  • Immunocytochemical techniques were employed.
  • Isolation of gp60 from human plasma and PBL culture supernatants.

Main Results:

  • gp60 was localized in human hepatocytes, platelets, and a subpopulation of peripheral blood lymphocytes (PBL).
  • gp60 is identical to a 60,000 MW IgG-binding protein (60-IBF) from PBL supernatants.
  • gp60 is distinct from Fc gamma RI, Fc gamma RII, and Fc gamma RIII.

Conclusions:

  • gp60 is a novel IgG Fc-binding protein found in human hepatocytes, platelets, and PBL.
  • gp60 represents a distinct entity from known Fc gamma receptors, suggesting unique functions.

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