Related Experiment Video

Updated: Aug 11, 2026

Use of a Monocyte Monolayer Assay to Evaluate Fcγ Receptor-mediated Phagocytosis
06:27

Use of a Monocyte Monolayer Assay to Evaluate Fcγ Receptor-mediated Phagocytosis

Published on: January 2, 2017

Influence of interleukin-4 and platelet-activating factor on the Fc epsilon RII/CD23 expression on human monocytes

N Paul-Eugène1, B Dugas, S Picquot

  • 1Institut Henri Beaufour, Les Ulis, France.

Journal of Lipid Mediators
|March 1, 1990
PubMed

Insights

Platelet-activating factor (PAF) increases the expression of the IgE receptor (Fc epsilon RII/CD23) on monocytes. This finding suggests a potential role for PAF in allergic diseases.

Area of Science:

  • Immunology
  • Cell Biology
  • Allergy Research

Background:

  • The low-affinity receptor for IgE (Fc epsilon RII/CD23) plays a role in allergic responses.
  • Interleukin-4 (IL-4) is known to induce Fc epsilon RII/CD23 expression on monocytes.

Purpose of the Study:

  • To investigate the effect of platelet-activating factor (PAF) on Fc epsilon RII/CD23 expression in human monocytes.
  • To compare the effects of PAF and IL-4 on Fc epsilon RII/CD23 expression.

Main Methods:

  • Human monocytes were treated with recombinant IL-4 or PAF.
  • Fc epsilon RII/CD23 expression was assessed using flow cytometry.
  • The effects of PAF antagonists (BN 52021, BN 50730) and anti-IL-4 antiserum were evaluated.

Main Results:

  • Both IL-4 and PAF induced Fc epsilon RII/CD23 expression on human monocytes.
  • PAF-induced expression was time- and dose-dependent, with maximal effects at 1 microM PAF after 24 hours.
  • PAF-induced expression was inhibited by PAF antagonists, but not by anti-IL-4 antiserum.
  • IL-4-induced expression was abolished by anti-IL-4 antiserum and slightly reduced by PAF antagonists.

Conclusions:

  • PAF significantly regulates Fc epsilon RII/CD23 expression on human monocytes.
  • These findings suggest a novel role for PAF in the context of allergic diseases.

More Related Videos

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes11:48

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry12:36

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

Related Concept Videos

Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...