Related Experiment Video

Updated: Aug 8, 2026

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
10:32

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Published on: June 5, 2015

Do infections induce monoclonal immunoglobulin components?

H Haas1, S Anders, G W Bornkamm

  • 1Research Institute, Borstel, FRG.

Clinical and Experimental Immunology
|September 1, 1990
PubMed

Insights

Monoclonal immunoglobulins (MC) were incidentally found in infections like visceral leishmaniasis and CMV. Further research is needed to understand the high incidence of MC in these specific infections.

Area of Science:

  • Infectious Diseases
  • Immunology
  • Clinical Chemistry

Background:

  • Monoclonal immunoglobulins (MC) are sometimes incidentally found in patients with infections.
  • The prevalence and significance of MC in various infectious diseases require systematic investigation.

Purpose of the Study:

  • To systematically investigate the presence of monoclonal immunoglobulin components (MC) in patients with specific infections.
  • To determine the incidence of MC in visceral leishmaniasis, cytomegalovirus (CMV) infection, echinococcosis, and infectious mononucleosis.

Main Methods:

  • Isoelectric focusing with immunoblotting was employed to detect MC.
  • The detection limit for MC was established at 0.1 mg/ml.
  • Patients with visceral leishmaniasis, CMV, echinococcosis, and infectious mononucleosis were analyzed.

Main Results:

  • MC were detected in 16/20 leishmaniasis patients and 8/18 CMV patients.
  • Only one echinococcosis patient and no infectious mononucleosis patients showed MC.
  • MC were generally transient, and a subset in leishmaniasis patients bound to leishmania antigens.

Conclusions:

  • A high incidence of MC was observed in visceral leishmaniasis and CMV infections.
  • The specificity of most detected MC remains undetermined.
  • Further research is necessary to elucidate the mechanisms behind MC occurrence in CMV and leishmaniasis.

More Related Videos

Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses10:10

Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses

Published on: November 16, 2016

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis08:44

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

Published on: September 7, 2022

Related Concept Videos

Humoral Immune Responses01:36

Humoral Immune Responses

Overview
Antibody Structure01:10

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Disorders of Leukocytes01:27

Disorders of Leukocytes

Leukocyte disorders can lead to either leukopenia, characterized by an abnormally low leukocyte count, or leukocytosis, marked by a very high leukocyte number.
Leukopenia may result from bone marrow disorders, autoimmune diseases, and infectious diseases. For example, conditions such as multiple myeloma and aplastic anemia can impair the bone marrow's ability to produce adequate leukocytes. Similarly, autoimmune diseases like lupus and viral infections such as HIV can prompt the immune system...
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Antibody Structure and Classes01:25

Antibody Structure and Classes

Antibodies, also known as immunoglobulins, are produced by B cells in response to foreign substances, such as bacteria and viruses. These proteins are critical for recognizing and neutralizing these substances, protecting the body from potential harm.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.