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A simple micro-photometric method for urinary iodine determination

Gabriele Grimm1, Heidelinde Lindorfer, Heidi Kieweg

  • 1Department of Laboratory Medicine, Medical University of Vienna and the General Hospital of the City of Vienna, Vienna, Austria. gabriele.grimm@meduniwien.ac.at

Clinical Chemistry and Laboratory Medicine
|June 28, 2011
PubMed

Insights

A new photometric test accurately measures urinary iodide concentration (UIC) for assessing iodine status and thyroid health. This simple method is faster than traditional techniques, making it suitable for clinical labs.

Area of Science:

  • Clinical Chemistry
  • Endocrinology
  • Analytical Chemistry

Background:

  • Urinary iodide concentration (UIC) is a key indicator of nutritional iodine status.
  • Accurate UIC measurement is crucial for managing thyroid conditions and radio-iodine therapies.
  • Existing methods for UIC analysis can be time-consuming and require specialized equipment.

Purpose of the Study:

  • To establish a simple and rapid photometric test for measuring urinary iodide concentration (UIC).
  • To provide a practical method for evaluating iodine status in clinical settings.
  • To offer an alternative to complex methods like ICP-MS for routine UIC analysis.

Main Methods:

  • Urine samples from 200 patients were analyzed using a modified Sandell-Kolthoff (SK) reaction.
  • Samples underwent pre-treatment with ammonium persulfate at 95°C.
  • The photometric SK test was validated against inductively coupled plasma mass spectrometry (ICP-MS).

Main Results:

  • The photometric SK test showed excellent correlation with ICP-MS (r=0.962).
  • The method achieved a functional sensitivity of 20 μg/L.
  • Median UIC in patient samples was 110 μg/L, with 90% below 210 μg/L.

Conclusions:

  • The modified SK-test provides accurate UIC measurements in approximately 90 minutes.
  • This photometric method is significantly faster than ICP-MS (27 hours).
  • The test is suitable for clinical laboratories due to its simplicity and use of basic equipment.
Abstract

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