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Updated: Aug 11, 2026
Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
Published on: November 8, 2011
[Large granular lymphocyte leukemia containing oligoclonal EB viral DNA]
1Second Department of Internal Medicine, Kyoto Prefectural University of Medicine.
Insights
This study identifies a rare case of lymphoproliferative disorder of granular lymphocytes (LDGL) in a female patient. Analysis suggests multiple clones, with chromosome abnormalities aiding in diagnosis.
Area of Science:
- Hematology
- Immunology
- Oncology
Background:
- Lymphoproliferative disorder of granular lymphocytes (LDGL) is a rare hematologic malignancy.
- Diagnosing LDGL can be challenging due to difficulties in demonstrating clonal origins.
Observation:
- A 32-year-old female presented with abdominal fullness, jaundice, and edema.
- Peripheral blood revealed leukocytosis with 84% leukemic cells; bone marrow showed 63.6% leukemic cells.
- Leukemic cells expressed CD2, OKIa1, NKH-1, exhibited NK and ADCC activity, but lacked T cell and NK cell markers (CD3, CD4, CD8, CD16).
- T cell receptor and immunoglobulin genes were in germline configuration, suggesting a non-T, non-B cell origin, likely NK cell lineage.
Findings:
- Despite polyclonal Epstein-Barr Virus (EBV) genome analysis, a consistent chromosomal abnormality (47, XX, +3) was identified.
- The findings suggest the presence of multiple clones, with chromosomal analysis detecting one clone.
- The study highlights the utility of EBV genome analysis and chromosomal studies in diagnosing LDGL.
Implications:
- This case expands the understanding of LDGL heterogeneity.
- It underscores the importance of integrating cytogenetic and molecular analyses for accurate LDGL diagnosis.
- The findings may guide future diagnostic strategies for similar hematologic malignancies.
Abstract:
A 32-year-old female was admitted to our hospital because of abdominal fullness, jaundice and pretibial edema in November, 1987. Leukocyte count of peripheral blood showed 13300/microliters with 84% leukemic cells and bone marrow was normocellular with 63.6% leukemic cells. Leukemic cells had azurophilic large granules with basophilic cytoplasm and positive for CD 2, OKIa 1, NKH-1 and negative for CD 3, CD 4, CD 8, CD 16. T cell receptor (TCR) genes for beta, gamma and delta chain and immunoglobulin heavy chain gene were in germ line configuration. These cells also had natural killer (NK) activity and antibody dependent cell mediated cytotoxicity (ADCC) activity. These observations suggested that they were derived from NK cell lineage. It is often difficult to demonstrate their clonalities in lymphoproliferative disorder of granular lymphocytes (LDGL). In the present case, the analysis of EBV genome using termini probe demonstrated polyclonal bands, while we found constant chromosome abnormalities; 47 XX, +3. From these observations, this case was considered to have several clones, and one of which could be detected by chromosome analysis. The analysis of EBV genome using termini probe may be useful to demonstrate their clonalities in LDGL in addition to conventional chromosome analysis.
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