Related Experiment Video

Updated: May 31, 2026

Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma
10:52

Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma

Published on: March 30, 2018

A minority of concurrent monoclonal lymphocytes and plasmacytic cells sharing light chains are genetically related in

Barbara K Zehentner1, Jevon A Cutler, Wayne K Fritschle

  • 1HematoLogics Inc., 3161 Elliott Ave, Seattle, WA 98121, USA. barbara@hematologics.com

Leukemia Research
|July 15, 2011
PubMed

Insights

Flow cytometry and gene rearrangement analysis distinguish B-cell lymphomas and plasma cell populations. Identical genotypes confirm Waldenström

Area of Science:

  • Hematology
  • Molecular Biology
  • Immunology

Background:

  • Distinguishing between phenotypically distinct B-cell lymphoid and plasma cell populations is crucial for accurate diagnosis.
  • Simultaneous occurrence of these distinct cell populations presents diagnostic challenges in hematologic malignancies.

Purpose of the Study:

  • To differentiate and characterize monoclonal B-cell lymphoid and plasma cell populations using combined flow cytometry and gene rearrangement analysis.
  • To establish genotypic criteria for defining Waldenström's/lymphoplasmacytic lymphoma (WM/LPL) and improving diagnostic confidence in related plasma cell disorders.

Main Methods:

  • Flow cytometric cell sorting was employed to isolate phenotypically distinct B-cell lymphoid and plasma cell populations.
  • Molecular gene rearrangement analysis was performed on sorted populations to determine monoclonality genotypes.
  • Genotypic profiles of lymphoid and plasma cell populations were compared across 38 specimens.

Main Results:

  • Identical monoclonality genotypes were found in 13 out of 38 specimens, with most exhibiting a Waldenström's/lymphoplasmacytic lymphoma (WM/LPL) phenotype.
  • Non-identical genotypic profiles were observed in 25 specimens, indicating distinct clonal origins for lymphoid and plasma cell populations.
  • WM/LPL phenotype was present in a significant proportion of both identical (majority) and non-identical (16/25) cases.

Conclusions:

  • An identical monoclonal genotype in B-lymphoid and plasma cells supports the definition of Waldenström's/lymphoplasmacytic lymphoma (WM/LPL) as a distinct entity.
  • Genotypically distinct populations aid in diagnosing concurrent B-lymphoid lymphomas with plasma cell dyscrasias like smoldering or multiple myeloma.
  • These combined techniques are essential for accurate diagnosis and prognosis in clinical studies involving monoclonal lymphoid and plasma cells.

More Related Videos

Bioprinting of Hydrogel Tumor Slices as a 3D Model for Mantle Cell Lymphoma08:31

Bioprinting of Hydrogel Tumor Slices as a 3D Model for Mantle Cell Lymphoma

Published on: September 12, 2025

Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking11:39

Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking

Published on: October 23, 2019

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
Lymphoid Cells and Tissues01:18

Lymphoid Cells and Tissues

Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
Lineage Commitment01:21

Lineage Commitment

Commitment is the  process whereby stem cells:
Disorders of Leukocytes01:27

Disorders of Leukocytes

Leukocyte disorders can lead to either leukopenia, characterized by an abnormally low leukocyte count, or leukocytosis, marked by a very high leukocyte number.
Leukopenia may result from bone marrow disorders, autoimmune diseases, and infectious diseases. For example, conditions such as multiple myeloma and aplastic anemia can impair the bone marrow's ability to produce adequate leukocytes. Similarly, autoimmune diseases like lupus and viral infections such as HIV can prompt the immune system...
Lampbrush Chromosomes01:51

Lampbrush Chromosomes

In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops resemble the...