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Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
Published on: November 13, 2021
Lateral flow assay for simultaneous detection of cellular- and humoral immune responses
Paul L A M Corstjens1, Claudia J de Dood, Jolien J van der Ploeg-van Schip
1Department of Molecular Cell Biology Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The Netherlands. P.Corstjens@LUMC.NL
Insights
A new assay using up-converting phosphor lateral flow (UCP-LF) technology can detect interleukin-10 (IL-10) for diagnosing mycobacterial diseases. This rapid, user-friendly method also enables multiplex detection of biomarkers, improving diagnostic capabilities.
Area of Science:
- Biotechnology
- Immunology
- Medical Diagnostics
Background:
- Mycobacterial diseases require improved diagnostic methods.
- Current diagnostic assays may lack speed or multiplexing capabilities.
Purpose of the Study:
- To develop a cytokine detection assay for diagnosing mycobacterial diseases.
- To create a multiplex assay for simultaneous biomarker detection.
Main Methods:
- Developed a lateral flow (LF) assay using up-converting phosphor (UCP) reporter technology to detect IL-10.
- Evaluated the assay with blood samples from leprosy patients.
- Explored multiplexing of IL-10 with IFN-γ and anti-PGL-I antibodies.
Main Results:
- Achieved IL-10 detection below 100 pg/mL in serum.
- Demonstrated quantitative correlation with ELISA (R²=0.92).
- Successfully performed multiplex detection of cytokines and simultaneous cytokine-antibody detection.
Conclusions:
- The UCP-LF IL-10 assay is a rapid, user-friendly alternative to IL-10 ELISAs.
- The assay is suitable for multiplex cytokine detection.
- It can be combined with antibody detection for simultaneous assessment of cellular and humoral immunity.
Objective:
The development of a cytokine detection assay suitable for detection of multiple biomarkers for improved diagnosis of mycobacterial diseases.
Design And Methods:
A lateral flow (LF) assay to detect IL-10 was developed utilizing the up-converting phosphor (UCP) reporter-technology. The assay was evaluated using blood samples of leprosy patients. Multiplex applications were explored targeting: 1) IL-10 and IFN-γ in assay buffer; 2) IL-10 and anti-phenolic glycolipid (PGL-I) antibodies in serum from leprosy patients.
Results:
Detection of IL-10 below the targeted level of 100pg/mL in serum was shown. Comparison with ELISA showed a quantitative correlation with R(2) value of 0.92. Multiplexing of cytokines and simultaneous detection of cytokine and antibody was demonstrated.
Conclusions:
The UCP-LF IL-10 assay is a user-friendly, rapid alternative for IL-10 ELISAs, suitable for multiplex detection of different cytokines and can be merged with antibody-detection assays to simultaneously detect cellular- and humoral immunity.
