A monoclonal antibody-based immunoperoxidase procedure for rapid detection of infectious bronchitis virus in infected...

Area of Science:

• Veterinary Pathology
• Immunohistochemistry
• Virology

Background:

• Infectious Bronchitis Virus (IBV) is a significant pathogen in poultry.
• Accurate and rapid detection of IBV antigen in tissues is crucial for disease diagnosis and control.
• Existing diagnostic methods may have limitations in sensitivity, speed, or cost.

Purpose of the Study:

• To develop and evaluate a monoclonal antibody (MAb)-based indirect immunoperoxidase (IP) procedure for detecting IBV antigen.
• To assess the sensitivity and timeliness of the IP technique in infected chicken tissues.
• To establish a convenient, economical, and permanent method for IBV diagnosis.

Main Methods:

• Application of a MAb-based indirect immunoperoxidase (IP) procedure.
• Detection of IBV antigen in frozen tissue sections from inoculated chickens.
• Treatment of chicken tissues with periodic acid to remove endogenous peroxidases.
• Evaluation of antigen-bearing cells and tissue morphology.

Main Results:

• IBV antigen was successfully detected in chorioallantoic membranes (CAMs) within 15 hours postinoculation.
• IBV antigen was detected in respiratory tissues of chickens within 96 hours postinoculation.
• The IP technique demonstrated high sensitivity, convenience, and cost-effectiveness.

Conclusions:

• The MAb-based IP procedure is a sensitive and efficient method for detecting IBV antigen in frozen chicken tissues.
• This technique allows for simultaneous assessment of viral antigen and tissue morphology.
• The IP-stained slides provide a permanent and valuable record for diagnostic and research purposes.