Related Experiment Video

Updated: May 29, 2026

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)
09:52

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)

Published on: April 19, 2013

Sequential chromatin immunoprecipitation assay and analysis

Ricardo B de Medeiros1

  • 1Department of Antibody Applications and Stem Cells, R&D Systems, Inc., Minneapolis, MN, USA. ricardo.demedeiros@rndsystems.com

Methods in Molecular Biology (Clifton, N.J.)
|September 14, 2011
PubMed

Insights

Sequential chromatin immunoprecipitation (SeqChIP) assays enable studying multiple protein interactions or histone modifications at specific genomic sites. This method is fast, sensitive, and preserves antibody-antigen binding for accurate analysis.

Area of Science:

  • Molecular Biology
  • Genomics
  • Epigenetics

Background:

  • Investigating simultaneous protein interactions or histone modifications at specific genomic locations is crucial for understanding gene regulation.
  • Existing methods may face limitations in sensitivity, speed, or preserving molecular interactions during sequential analysis.

Purpose of the Study:

  • To present a refined Sequential Chromatin Immunoprecipitation (SeqChIP) protocol.
  • To highlight the protocol's efficiency, sensitivity, and ability to maintain antibody-antigen integrity.

Main Methods:

  • Sequential Chromatin Immunoprecipitation (SeqChIP) involving sequential antibody-based immunoprecipitation of chromatin.
  • Optimization for speed (3-4 hours) and sensitivity (detecting targets in ~20,000 cells).

Main Results:

  • The SeqChIP assay successfully identifies co-occupancy of multiple proteins or histone modifications at genomic sites.
  • Demonstrated preservation of antibody-antigen interactions throughout the sequential process.
  • Examples of solved protein complexes and interactions using SeqChIP are presented.

Conclusions:

  • SeqChIP offers a rapid, sensitive, and robust method for analyzing complex molecular interactions at the genome.
  • This technique advances the study of protein-protein interactions and epigenetic modifications in a simultaneous manner.

More Related Videos

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass14:29

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Published on: May 1, 2013

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin24:02

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

Related Concept Videos

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...