Related Experiment Video
Updated: May 28, 2026
A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection
Published on: June 15, 2018
[Serological detection of Cryptosporidium spp. infection in outpatients in Changchun]
Jun-Peng Song1, Ji-Xue Zhao, Hong Gao
1Key Laboratory for Zoonosis Research, Ministry of Education Changchun 130062, China.
Insights
A study on Cryptosporidium parvum found that the CP23 protein is a potential diagnostic marker. The developed ELISA test detected anti-CP23 IgG in 3.2% of individuals, with higher rates in older age groups.
Area of Science:
- Parasitology
- Immunology
- Molecular Biology
Context:
- Cryptosporidium parvum is a significant cause of diarrheal disease globally.
- Accurate diagnostic tools are crucial for managing cryptosporidiosis.
- The CP23 protein is a potential antigen for Cryptosporidium parvum detection.
Purpose:
- To express and purify the recombinant CP23 protein from Cryptosporidium parvum.
- To evaluate the immunoreactivity of the recombinant CP23 protein.
- To develop and validate an indirect ELISA for detecting anti-CP23 IgG antibodies in human sera.
Summary:
- Recombinant CP23 protein was successfully expressed and purified.
- Western blotting confirmed the specificity of CP23 recognition by positive human and calf sera.
- An indirect ELISA detected anti-CP23 IgG in 3.2% of the study population (65/2046).
- Seropositive rates varied by age, with the 71-80 age group showing the highest prevalence (8.6%).
Impact:
- The study validates CP23 as a specific antigen for Cryptosporidium parvum.
- The developed ELISA offers a potential diagnostic method for cryptosporidiosis.
- Findings highlight the importance of age in seroprevalence studies of parasitic infections.
Abstract:
CP23 gene of Cryptosporidium parvum was expressed in Escherichia coli, and the recombinant protein was purified. Its immunoreactivity was analyzed by Western blotting. Serum samples were collected from outpatients of different ages from August to November, 2010 in Changchun. Indirect ELISA was established to detect the anti-CP23 IgG in sera. Western blotting analysis indicated that the recombinant CP23 protein was recognized by sera from Cryptosporidium panum-infected calves and positive human sera, but not recognized by sera of mice infected with Schistosoma japonicum, sera from falciparum malaria patients and negative human sera. The overall anti-CP23 IgG positive rate was 3.2% (65/2 046). The seropositive rate was 2.7% (28/1 036) in men and 3.7% (37/1 010) in women (P > 0.05). The seropositive rates were significantly different among age groups (P < 0.05), and the age group of 71-80 had the highest positive rate (8.6%, 13/152).