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Updated: May 27, 2026
Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species
Published on: May 19, 2018
Comparative studies on the distribution and population of immunocompetent cells in bovine hemal node, lymph node and
Weidong Zhang1, Tetsuo Nasu, Yoshinao Z Hosaka
1Department of Veterinary Anatomy, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
Insights
Bovine hemal nodes contain abundant immunocompetent cells, suggesting a significant role in cellular and humoral immunity, similar to lymph nodes and spleens. This research highlights the immune function of cattle hemal nodes.
Area of Science:
- Veterinary Immunology
- Comparative immunology
- Bovine immunology
Background:
- The immune system's complexity involves various lymphoid organs.
- Understanding cell distribution in these organs is crucial for immunity.
- Bovine hemal nodes' immune function remains less understood compared to lymph nodes and spleens.
Purpose of the Study:
- To comparatively analyze the distribution and population of immunocompetent cells in bovine hemal nodes, mesenteric lymph nodes, and spleens.
- To elucidate the specific roles of hemal nodes in bovine immunity.
Main Methods:
- Immunohistochemistry
- Flow cytometry
- Comparative analysis of cell populations (CD8+, CD172a+, γδ T cells, CD21+, MHC class II+).
Main Results:
- Hemal nodes and spleens showed higher populations of CD8+ cells and γδ T cells compared to mesenteric lymph nodes.
- Hemal nodes and mesenteric lymph nodes had higher populations of CD21+ and MHC class II+ cells than spleens.
- Germinal centers were prevalent in the cortex and white pulp of all examined lymphoid organs.
Conclusions:
- Bovine hemal nodes play a significant role in both cellular and humoral immunity.
- Hemal nodes exhibit immune functions comparable to lymph nodes and spleens in cattle.
- The distribution of immunocompetent cells indicates a multifaceted immune role for hemal nodes.
Abstract:
The distribution and population of immunocompetent cells in bovine hemal node, mesenteric lymph node and spleen were analyzed comparatively by immunohistochemistry and flow cytometry. Many CD8(+) cells, CD172a(+) cells and γδ T cells were found in the lymphatic cord along the sinus of the hemal node and the splenic red pulp. A few CD8(+) cells and γδ T cells were distributed diffusely in the paracortex and medullary cord of the mesenteric lymph node. Many germinal centers were recognized in the lymphatic regions such as the cortex and white pulp of these lymphoid organs. The populations of CD8(+) cells and γδ T cells in the hemal node and the spleen were higher than those of the mesenteric lymph node. In addition, the populations of CD21(+) cells and MHC class II(+) cells in the hemal node and the mesenteric lymph node were higher than those of the spleen. The results suggest that the hemal node has an important role in both cellular and humoral immunity as well as the lymph node and the spleen in cattle.
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