Related Experiment Video

Updated: Aug 11, 2026

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
11:44

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

Published on: January 24, 2016

Sarcolectin and interferon in the regulation of cell growth

F Chany-Fournier1, P H Jiang, C Chany

  • 1Institut National de la Santé et de la Recherche Médicale, Hôpital Saint Vincent de Paul, Paris, France.

Journal of Cellular Physiology
|October 1, 1990
PubMed

Insights

Sarcolectin, a 65-kd protein, inhibits interferon-dependent protein synthesis and restores cell growth. This endolectin plays a role in a triangular growth-regulatory cycle, balancing cell development.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Background:

  • Sarcolectin is an endolectin found in various vertebrate tissues, including brain and placenta.
  • It is a 65-kd protein that exhibits cell agglutination and affinity for simple sugars.

Purpose of the Study:

  • To investigate the role of sarcolectin in regulating cellular processes, particularly its interaction with interferon (IFN) and cell growth.
  • To elucidate the mechanism by which sarcolectin affects IFN-dependent protein synthesis and cellular refractoriness.

Main Methods:

  • Purification of sarcolectin to electrophoretical homogeneity.
  • Assaying sarcolectin's effects on cell agglutination, sugar affinity, and inhibition of interferon-dependent protein synthesis.
  • Investigating the interplay between sarcolectin, Poly(I).Poly(C), and interferon (IFN) in regulating cell growth.

Main Results:

  • Sarcolectin inhibits the synthesis of interferon (IFN)-dependent secondary proteins and restores cells to their original state.
  • Sarcolectin abolishes cellular refractoriness to repeated IFN induction and restores cell growth capacity.
  • A balance between sarcolectin and IFN concentrations determines the outcome of cell growth regulation.

Conclusions:

  • Sarcolectin plays a critical role in a triangular growth-regulatory cycle involving Poly(I).Poly(C) and IFN.
  • This cycle is proposed to maintain balance during differentiated normal tissue development.
  • Sarcolectin's ability to initiate cell growth after IFN-induced blockage highlights its significance in cellular regulation.

More Related Videos

Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses10:10

Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses

Published on: November 16, 2016

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice08:09

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Published on: March 24, 2017

Related Concept Videos

Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
Molecular Factors Affecting Cell Division01:27

Molecular Factors Affecting Cell Division

Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...