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Updated: May 26, 2026
Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation
Published on: November 26, 2018
[Analysis of immunophenotype characteristics in 109 cases of B lineage chronic B lymphoid leukemia]
Ai-Qing Wang1, Mei-Ju Geng, Ming-Qing Zhu
1Soochow University Experiment Center, Suzhou, Jiangsu Province, China.
Insights
Immunophenotyping B-cell chronic lymphoid leukemia (B-CLL) using flow cytometry aids diagnosis and minimal residual disease detection. Further research is needed to link B-CLL immunophenotypic traits with patient prognosis.
Area of Science:
- Hematology
- Immunology
- Oncology
Context:
- B-cell chronic lymphoid leukemia (B-CLL) is a common lymphoid malignancy.
- Accurate immunophenotypic characterization is crucial for B-CLL diagnosis and management.
- Multiparameter flow cytometry is a standard technique for immunophenotyping.
Purpose:
- To investigate the immunophenotypic profile of B-CLL cases.
- To establish evidence for B-CLL diagnosis, therapy, and minimal residual disease detection.
- To explore potential correlations between immunophenotype and prognosis.
Summary:
- 109 B-CLL cases were analyzed using two/three-color multiparameter flow cytometry.
- All cases expressed CD19; high positivity for CD20, CD22, CD23; none expressed CD10.
- CD5 was expressed in 86.23% of cases; ZAP-70 in 12/50 patients. FMC-7 and CD38 expression were 28.60% and 36.20% respectively.
Impact:
- Immunophenotypic data are valuable for B-CLL diagnosis and monitoring minimal residual disease.
- The findings support the utility of flow cytometry in B-CLL management.
- Further studies are required to elucidate the prognostic significance of B-CLL immunophenotypic characteristics.
Abstract:
This study was aimed to investigate the immunophenotypic characteristics of 109 cases of B-cell chronic lymphoid leukemia (B-CLL) so as to provide evidences for the diagnosis and therapy of B-CLL, and for the detection of the minimal residual disease and its prognosis. Immunophenotyping was performed in 109 patients of B-CLL by two/three color multiparameter flow cytometry analysis using a panel of monoclonal antibodies. The results showed that in 109 cases of B-CLL, all cases expressed CD19, the positive ratios of other B lineage antigen such as CD20, CD22 and CD23 were 95.40%, 94.50%, 86.20% respectively. None of the B-CLL cases expressed CD10. The expression ratio of FMC-7 and CD38 in 105 cases of B-CLL were 28.60% and 36.20%. Among the B-CLL cases the CD5(+) cells amounted to 86.23%, CD5(-) cells amounted to 13.76%, ZAP-70 protein was expressed in 12 out of 50 patients. It is concluded that immunophenotypic data are very useful for the diagnosis and detection of minimal residual disease of B-CLL, and the relationship between the immunophenotypic characteristics and the prognosis of B-CLL needs further study.
