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Th17 Inflammation Model of Oropharyngeal Candidiasis in Immunodeficient Mice
Published on: February 18, 2015
Murine bone marrow-derived dendritic cells and T-cell activation by Candida albicans
Joanne Gibson1, Neil A R Gow, Simon Y C Wong
1School of Medicine and Dentistry, University of Aberdeen, Aberdeen, UK.
Insights
Investigating dendritic cell (DC) responses to Candida albicans reveals how these immune cells guide T-cell proliferation and cytokine production, crucial for effective immune responses against fungal pathogens.
Area of Science:
- Immunology
- Cell Biology
Background:
- Dendritic cells (DCs) are key antigen-presenting cells that bridge innate and adaptive immunity.
- DCs initiate T-cell responses by producing cytokines and presenting antigens.
- Understanding DC-pathogen interactions is vital for elucidating T-cell immunity induction.
Purpose of the Study:
- To describe methods for assessing T-cell responses to DCs stimulated by Candida albicans.
- To investigate the proliferative and cytokine responses of murine splenic T cells.
Main Methods:
- Utilizing mixed leukocyte reactions (MLRs).
- Co-culturing murine splenic T cells with bone marrow-derived dendritic cells (BMDCs).
- Stimulating BMDCs with Candida albicans components.
Main Results:
- The study outlines a methodology for evaluating T-cell responses.
- This approach allows for the analysis of T-cell proliferation and cytokine profiles.
- The results provide a framework for studying DC-mediated T-cell activation.
Conclusions:
- The described methods enable the study of dendritic cell-T cell interactions.
- This research contributes to understanding how DCs orchestrate pathogen-specific T-cell immunity.
- The findings are relevant for developing strategies against fungal infections.
Abstract:
Dendritic cells (DCs) detect and respond to microbes or their components by producing cytokines and other molecules that can activate the proliferation and differentiation pathways of T cells. Investigation of DC responses to pathogens would thus provide important insights into how T-cell responses most appropriate for the pathogen are induced. Here, we describe methods for the use of mixed leukocyte reactions, to determine the proliferative and cytokine responses of murine splenic T cells in response to co-culture with bone marrow-derived DCs stimulated with Candida albicans.
