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A monoclonal-antibody-defined adhesion-related antigen on bovine neutrophils is required for neutrophil aggregation

P N Bochsler1, M Doré, N R Neilsen

  • 1Department of Pathology, College of Veterinary Medicine, Cornell University, Ithaca, New York.

Inflammation
|October 1, 1990
PubMed

Insights

Researchers identified a novel surface molecule on bovine neutrophils (PMNs) using a mouse antibody. This molecule

Area of Science:

  • Immunology
  • Veterinary Science
  • Cell Biology

Background:

  • Leukocyte surface adhesion molecules regulate critical cellular events like extravasation and aggregation.
  • Understanding these molecules in different species, like cattle, is crucial for veterinary immunology.

Purpose of the Study:

  • To identify and characterize a specific antigen on bovine neutrophils (PMNs) using a mouse anti-human monoclonal antibody (MAb 60.3).
  • To investigate the functional role of this antigen in PMN adhesion and aggregation.

Main Methods:

  • Indirect immunofluorescence and flow cytometry were used to detect and quantify the antigen on bovine PMNs.
  • The effect of temperature and stimuli (zymosan-activated serum, phorbol 12-myristate 13-acetate, lipopolysaccharide) on antigen expression was analyzed.
  • Monoclonal antibody 60.3 was used to inhibit PMN-PMN aggregation assays.

Main Results:

  • Monoclonal antibody 60.3 identified a surface antigen on both resting and stimulated bovine PMNs.
  • Antigen expression increased with temperature and stimulation by zymosan-activated serum and phorbol 12-myristate 13-acetate, but not by bacterial lipopolysaccharide.
  • Preincubation with MAb 60.3 significantly inhibited phorbol 12-myristate 13-acetate-induced PMN aggregation.

Conclusions:

  • Bovine neutrophils express a surface molecule recognized by MAb 60.3 that likely plays a role in adhesion-related functions.
  • Stimulation can enhance the surface expression of this adhesion molecule on bovine PMNs.

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