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Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum
Published on: December 13, 2017
Cardiac troponin I: a case study in rational antibody design for human diagnostics
P J Conroy1, R J O'Kennedy, S Hearty
1Biomedical Diagnostics Institute, National Centre for Sensor Research and School of Biotechnology, Dublin City University, Dublin 9, Ireland.
Insights
This study presents a rational method for creating epitope-specific antibodies for early cardiovascular disease detection. These advanced biorecognition elements improve the performance of immuno-diagnostic devices.
Area of Science:
- Biotechnology
- Immunodiagnostics
- Cardiovascular Disease Research
Background:
- In vitro diagnostic (IVD) platforms are crucial for rapid disease status determination.
- The clinical performance of antibody-based IVDs relies heavily on the biorecognition entity for analytical specificity.
- Optimizing antibodies is essential for specific applications in immuno-IVD devices.
Purpose of the Study:
- To demonstrate a rational, knowledge-driven approach for generating epitope-specific antibodies.
- To develop superior biorecognition elements for early cardiovascular disease detection.
- To discuss high-throughput (HT) strategies for antibody library mining.
Main Methods:
- Utilizing combinatorial antibody generation and molecular evolution strategies.
- Employing high-throughput surface plasmon resonance (SPR) screening tools.
- Applying a rational, knowledge-driven methodology for antibody optimization.
Main Results:
- Successfully generated epitope-specific antibodies for cardiovascular disease early detection.
- Demonstrated the efficacy of a tailored antibody optimization approach.
- Highlighted the potential of HT strategies for mining large antibody libraries.
Conclusions:
- The developed methodologies expedite the creation of superior biorecognition elements for cardiovascular disease diagnostics.
- Rational antibody generation and optimization are key to enhancing immuno-IVD performance.
- High-throughput screening is vital for efficiently mining antibody libraries for diagnostic applications.
Abstract:
In vitro diagnostic (IVD) platforms provide rapid and accurate determination of disease status. The clinical performance of antibody-based diagnostic platforms is paramount as the information provided often informs the medical intervention taken and, ultimately, the patient's outcome. Breaking down such an immuno-IVD device into its component elements, the biorecognition entity is key to the analytical specificity of the test. Furthermore, tailored optimisation of the antibody is often necessary to impart the desired biophysical properties for the specific application. This tailoring is now widely facilitated by advances in combinatorial approaches to antibody generation, molecular evolution strategies and the availability of truly high-throughput (HT), refined surface plasmon resonance-based screening tools. In this paper, we demonstrate a rational, knowledge-driven approach to the generation of epitope-specific antibodies for the early detection of cardiovascular disease, discuss the merits of the approaches taken and offer a perspective on HT strategies to mining large antibody libraries. These results highlight the expedience of such methodologies for the development of truly superior cardiovascular disease biorecognition elements.
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